Abstract
Monitoring of the Epstein-Barr virus-DNA load is frequently used to identify patients at risk for post-transplant lymphoproliferative disease (PTLD). Epstein-Barr virus DNA can be measured in the plasma and whole blood serum compartments. We compared levels of Epstein-Barr virus DNA in whole blood and plasma using a real-time TaqMan polymerase chain reaction assay in 100 consecutive paired whole blood and plasma samples from 25 lung and heart-lung transplant recipients with detectable whole blood Epstein-Barr virus-DNA load (>2,000 copies/ml). A correlation (r2) of 0.58 (p < 0.001) was observed between both measurements, whereas of the positive whole blood samples (>2,000 copies/ml), only 17 samples (18%) were also positive in plasma. These results indicate that by virtue of its sensitivity, whole blood rather than plasma may be the preferable specimen for the detection of Epstein-Barr virus DNA in lung transplant recipients.
References
Sep 1, 1994·Leukemia & Lymphoma·J L McKnightP S Joseph
Feb 27, 2001·Blood·S J StevensJ M Middeldorp
Mar 16, 2001·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·E A. VerschuurenJ M. Middeldorp
Apr 3, 2001·Journal of Clinical Microbiology·S J StevensJ M Middeldorp
Oct 2, 2001·Transplantation·H J WagnerP Bucsky
Aug 3, 2002·Leukemia & Lymphoma·Servi J C StevensJaap M Middeldorp
Nov 8, 2003·Journal of Clinical Microbiology·Robert M WadowskyDavid Rowe
Dec 16, 2003·The Journal of Infectious Diseases·Linda CarpentierCaroline Alfieri
May 6, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Samira Fafi-KremerPatrice Morand
Aug 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Timothy C LeeJohn A Goss
Dec 21, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Christian BendenMartin J Elliott
Jun 1, 2006·Transplant Infectious Disease : an Official Journal of the Transplantation Society·T D BartonA P Limaye
Sep 19, 2008·Journal of Clinical Microbiology·Fausto BaldantiFranco Locatelli
Citations
Apr 9, 2010·Clinical Microbiology Reviews·Margaret L Gulley, Weihua Tang
Sep 19, 2008·Journal of Clinical Microbiology·Fausto BaldantiFranco Locatelli
Feb 9, 2013·Expert Review of Clinical Immunology·Stephanie Ruf, Hans-Joachim Wagner
Dec 21, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·S RufH J Wagner
Apr 8, 2010·Pediatric Clinics of North America·Thomas G GrossAngela Punnett
Dec 3, 2014·Journal of Virological Methods·Margit HübnerHarald H Kessler
Jun 17, 2008·The Journal of Molecular Diagnostics : JMD·Margaret L Gulley, Weihua Tang
May 22, 2008·Reviews in Medical Virology·Hiroshi KimuraYukihiro Nishiyama
Jun 12, 2014·Acta Clinica Belgica·L CattoirE Padalko
Aug 13, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Kirsten SchafferJeff Connell
Jan 25, 2013·Clinical & Developmental Immunology·A E GreijerJ M Middeldorp
May 10, 2016·Revista Argentina de microbiología·María Dolores FellnerMaría A Picconi
Jan 31, 2008·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Megan K DishopFrances V White
Jul 22, 2009·Current Opinion in Organ Transplantation·David W Zaas