Quantification of Epstein-Barr virus-DNA load in lung transplant recipients: a comparison of plasma versus whole blood

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
Nicolaas A BakkerBouke G Hepkema

Abstract

Monitoring of the Epstein-Barr virus-DNA load is frequently used to identify patients at risk for post-transplant lymphoproliferative disease (PTLD). Epstein-Barr virus DNA can be measured in the plasma and whole blood serum compartments. We compared levels of Epstein-Barr virus DNA in whole blood and plasma using a real-time TaqMan polymerase chain reaction assay in 100 consecutive paired whole blood and plasma samples from 25 lung and heart-lung transplant recipients with detectable whole blood Epstein-Barr virus-DNA load (>2,000 copies/ml). A correlation (r2) of 0.58 (p < 0.001) was observed between both measurements, whereas of the positive whole blood samples (>2,000 copies/ml), only 17 samples (18%) were also positive in plasma. These results indicate that by virtue of its sensitivity, whole blood rather than plasma may be the preferable specimen for the detection of Epstein-Barr virus DNA in lung transplant recipients.

References

Mar 16, 2001·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·E A. VerschuurenJ M. Middeldorp
May 6, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Samira Fafi-KremerPatrice Morand
Aug 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Timothy C LeeJohn A Goss
Dec 21, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Christian BendenMartin J Elliott
Jun 1, 2006·Transplant Infectious Disease : an Official Journal of the Transplantation Society·T D BartonA P Limaye

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Citations

Feb 9, 2013·Expert Review of Clinical Immunology·Stephanie Ruf, Hans-Joachim Wagner
Apr 8, 2010·Pediatric Clinics of North America·Thomas G GrossAngela Punnett
Dec 3, 2014·Journal of Virological Methods·Margit HübnerHarald H Kessler
Jun 17, 2008·The Journal of Molecular Diagnostics : JMD·Margaret L Gulley, Weihua Tang
May 22, 2008·Reviews in Medical Virology·Hiroshi KimuraYukihiro Nishiyama
Aug 13, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Kirsten SchafferJeff Connell
May 10, 2016·Revista Argentina de microbiología·María Dolores FellnerMaría A Picconi
Jan 31, 2008·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Megan K DishopFrances V White
Jul 22, 2009·Current Opinion in Organ Transplantation·David W Zaas

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