Quantification of exercise-regulated ubiquitin signaling in human skeletal muscle identifies protein modification cross talk via NEDDylation.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Benjamin L ParkerDavid E James

Abstract

The maintenance of muscle function is extremely important for whole body health and exercise is essential to this process. The ubiquitin-proteasome system (UPS) is required for muscle adaptation following exercise but little is known about acute posttranslational regulation and proteome remodeling during and after high-intensity exercise. Here, we used quantitative proteomics to study ubiquitin signaling dynamics in human skeletal muscle biopsies from healthy males before, during, and after a single bout of high-intensity exercise. Exercise resulted in a marked depletion of protein ubiquitylation in the vastus lateralis muscle consistent with proteasome activation. This was also associated with acute posttranslational modification of protein abundance. Integration of these data with phosphoproteomics identified co-regulated proximal modifications suggesting a cross talk between phosphorylation and ubiquitylation. We also identified additional protein modification cross talk and showed acute activation of protein NEDDylation. In vitro experiments revealed that cAMP-dependent activation of the proteasome requires NEDD8, an ubiquitin-like protein involved in protein NEDDylation, to maintain cellular protein ubiquitylation levels. ...Continue Reading

References

Dec 6, 1976·European Journal of Applied Physiology and Occupational Physiology·G Haralambie, A Berg
Oct 1, 1987·Medicine and Science in Sports and Exercise·G L DohmG J Kasperek
Nov 15, 1994·Biochemical and Biophysical Research Communications·H S Thompson, S P Scordilis
Jul 1, 1997·The American Journal of Physiology·S M PhillipsR R Wolfe
Jan 14, 1999·The American Journal of Physiology·S M PhillipsR R Wolfe
Oct 30, 2001·Behavioural Brain Research·Y BenjaminiI Golani
Dec 20, 2002·Canadian Journal of Physiology and Pharmacology·S M PhillipsM A Tamopolsky
Dec 4, 2003·Medicine and Science in Sports and Exercise·Darryn S WilloughbyLemuel Taylor
May 20, 2004·American Journal of Physiology. Endocrinology and Metabolism·M Sheffield-MooreR R Wolfe
Sep 15, 2004·The Journal of Biological Chemistry·Xin YeJ Wade Harper
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Oct 24, 2007·Nature Methods·Lukas KällMichael J MacCoss
May 1, 2008·American Journal of Physiology. Endocrinology and Metabolism·Hemang ParikhLeif C Groop
Sep 4, 2010·Cell·Jung-Shin LeeAli Shilatifard
Sep 13, 2011·Molecular Cell·Woong KimSteven P Gygi
Nov 15, 2011·Journal of Proteome Research·Thomas TausKarl Mechtler
Feb 9, 2013·Molecular Biology and Evolution·Andrew Paul HutchinsDiego Miranda-Saavedra
Nov 16, 2013·EMBO Reports·John R LydeardJ Wade Harper
Dec 23, 2014·Nature Reviews. Molecular Cell Biology·Radoslav I EnchevMatthias Peter
Dec 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Sudarsanareddy LokireddyAlfred Lewis Goldberg
Mar 18, 2016·Diabetes·Marissa J SchaferNathan K LeBrasseur
Mar 26, 2016·Cell Research·Kirby N Swatek, David Komander
Aug 12, 2016·American Journal of Physiology. Cell Physiology·Philippe A BilodeauSimon S Wing
Oct 28, 2016·Cell Systems·Christopher M RoseSteven P Gygi
Dec 25, 2016·Cell Metabolism·Gelsomina MansuetoAndrea Ballabio
Feb 27, 2017·Journal of Molecular Biology·Pavel FilipčíkPeter D Mace
Sep 6, 2018·Molecular and Cellular Biology·Rafeeq MirSanjeev Galande
Nov 6, 2018·Nucleic Acids Research·Yasset Perez-RiverolJuan Antonio Vizcaíno
Jan 7, 2019·European Journal of Applied Physiology·Luke A OlsenAndrew C Fry
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jordan J S VerPlankAlfred L Goldberg
Apr 5, 2019·Nature Communications·Yingyao ZhouSumit K Chanda

❮ Previous
Next ❯

Citations

Jul 1, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stuart J HeskethJatin G Burniston
Oct 8, 2020·The FEBS Journal·Tao Zou, Jiyan Zhang
Jan 21, 2021·Expert Review of Proteomics·Stuart J HeskethJatin G Burniston
Dec 22, 2020·Frontiers in Physiology·Alex P Seabright, Yu-Chiang Lai
Feb 1, 2021·Molecular & Cellular Proteomics : MCP·Craig A GoodmanPaul Gregorevic
May 27, 2021·American Journal of Physiology. Cell Physiology·Yusuke NishimuraYu-Chiang Lai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.