Quantification of Focal Outflow Enhancement Using Differential Canalograms

Investigative Ophthalmology & Visual Science
Ralitsa T LoewenNils A Loewen

Abstract

To quantify regional changes of conventional outflow caused by ab interno trabeculectomy (AIT). Gonioscopic, plasma-mediated AIT was established in enucleated pig eyes. We developed a program to automatically quantify outflow changes (R, package eye-canalogram, github.com) using a fluorescent tracer reperfusion technique. Trabecular meshwork (TM) ablation was demonstrated with fluorescent spheres in six eyes before formal outflow quantification with two-dye reperfusion canalograms in six additional eyes. Eyes were perfused with a central, intracameral needle at 15 mm Hg. Canalograms and histology were correlated for each eye. The pig eye provided a model with high similarity to AIT in human patients. Histology indicated ablation of TM and unroofing of most Schlemm's canal segments. Spheres highlighted additional circumferential and radial outflow beyond the immediate area of ablation. Differential canalograms showed that AIT caused an increase of outflow of 17 ± 5-fold inferonasally, 14 ± 3-fold superonasally, and also an increase in the opposite quadrants with a 2 ± 1-fold increase superotemporally, and 3 ± 3 inferotemporally. Perilimbal specific flow image analysis showed an accelerated nasal filling with an additional perili...Continue Reading

Citations

Apr 5, 2019·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ying HongNils A Loewen
Oct 16, 2018·Journal of Cataract and Refractive Surgery·Samir NazaraliPatrick Gooi
Sep 9, 2017·International Ophthalmology Clinics·Tavé van Zyl, Angela Turalba
May 23, 2017·F1000Research·Katherine FallanoNils A Loewen
Sep 26, 2017·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Tigran KostanyanNils A Loewen
Jan 29, 2019·Journal of Glaucoma·Anish AroraPatrick Gooi
May 4, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yalong DangNils A Loewen
May 31, 2018·Investigative Ophthalmology & Visual Science·Susannah WaxmanNils A Loewen
May 7, 2020·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Si ChenNils A Loewen
Nov 21, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yalong DangNils A Loewen
Apr 4, 2020·Scientific Reports·Fiona McDonnellW Daniel Stamer
Apr 5, 2018·Scientific Reports·Yalong DangNils A Loewen
Oct 17, 2019·Translational Vision Science & Technology·Kevin C SnyderGillian J McLellan
Aug 22, 2020·Translational Vision Science & Technology·Chen XinNingli Wang

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