Quantification of free coumarin and its liberation from glucosylated precursors by stable isotope dilution assays based on liquid chromatography-tandem mass spectrometric detection

Journal of Agricultural and Food Chemistry
Michael Rychlik

Abstract

A stable isotope dilution assay for the quantification of free coumarin and glucosylated coumarin precursors has been developed using [13C2]-coumarin as the internal standard. The doubly labeled coumarin was synthesized by reacting [13C2]-acetic anhydride with salicylic aldehyde and characterized by means of mass spectrometry and nuclear magnetic resonance (NMR) experiments. The specifity of liquid chromatography-tandem mass spectrometry enabled unequivocal determination and sensitive quantitation of the odorant. Because of the very simple extraction procedure, free coumarin could be analyzed within 1h. For quantification of total coumarin, the odorant was liberated from its precursors by an incubation with hydrochloric acid or beta-glucosidase. In analyses of breakfast cereals, the intra-assay coefficient of variation was 9.9% ( n = 5) for total coumarin. When coumarin was added to butter cookies at a level of 10 microg/kg, a recovery of 94.1% was found. Further addition studies revealed a detection limit of 2.9 microg/kg and a quantification limit of 8.6 microg/kg. Application of the stable isotope dilution assay to several plants, foods, and essential oils revealed high contents in cassia products and those foods in which ca...Continue Reading

Citations

Aug 6, 2013·Analytical and Bioanalytical Chemistry·Sonja KriegerOliver J Schmitz
Jan 16, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·S P CrispimUNKNOWN European Food Consumption Validation (EFCOVAL)
Nov 4, 2010·Journal of Fluorescence·Mirian MarcolanLucia Codognoto
Apr 12, 2016·International Journal of Pediatric Otorhinolaryngology·Deniz HancıCemal Cingi
Aug 6, 2011·Alimentary Pharmacology & Therapeutics·H E CampbellM C E Lomer
Dec 22, 2009·Molecular Nutrition & Food Research·Klaus AbrahamAlfonso Lampen
Apr 23, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Sleman KadanHilal Zaid
Oct 13, 2016·Journal of Agricultural and Food Chemistry·Juliane WagnerPeter Schieberle
Nov 12, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhiqin RenFeng Zhang
Dec 20, 2016·Journal of Agricultural and Food Chemistry·Juliane WagnerMichael Granvogl
Nov 18, 2017·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Daniel SandnerRalf G Berger
Dec 29, 2017·BMC Complementary and Alternative Medicine·Priyanga RanasinghePriyadarshani Galappaththy
Sep 6, 2008·Phytochemical Analysis : PCA
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eon-Joo Roh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.