PMID: 16636447Apr 26, 2006Paper

Quantification of genetically modified soybeans using a combination of a capillary-type real-time PCR system and a plasmid reference standard

Bioscience, Biotechnology, and Biochemistry
Akie ToyotaTamio Maitani

Abstract

Because the labeling of grains and feed- and foodstuffs is mandatory if the genetically modified organism (GMO) content exceeds a certain level of approved genetically modified varieties in many countries, there is a need for a rapid and useful method of GMO quantification in food samples. In this study, a rapid detection system was developed for Roundup Ready Soybean (RRS) quantification using a combination of a capillary-type real-time PCR system, a LightCycler real-time PCR system, and plasmid DNA as the reference standard. In addition, we showed for the first time that the plasmid and genomic DNA should be similar in the established detection system because the PCR efficiencies of using plasmid DNA and using genomic DNA were not significantly different. The conversion factor (Cf) to calculate RRS content (%) was further determined from the average value analyzed in three laboratories. The accuracy and reproducibility of this system for RRS quantification at a level of 5.0% were within a range from 4.46 to 5.07% for RRS content and within a range from 2.0% to 7.0% for the relative standard deviation (RSD) value, respectively. This system rapidly monitored the labeling system and had allowable levels of accuracy and precision.

References

Dec 22, 1999·Journal of Agricultural and Food Chemistry·S VollenhoferH Kroath
Jun 1, 2001·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·T MatsuokaA Hino
Mar 21, 2002·Journal of Agricultural and Food Chemistry·Takeshi MatsuokaAkihiro Hino
May 10, 2002·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hiroshi AkiyamaMasatake Toyoda
Mar 1, 2003·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hiroshi AkiyamaMasatake Toyoda
Mar 25, 2004·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Takahiro WatanabeTamio Maitani
May 27, 2004·Journal of Agricultural and Food Chemistry·Hsin-Ying Huang, Tzu-Ming Pan
Aug 5, 2004·Journal of Agricultural and Food Chemistry·Michael E RottMargaret J Green
Sep 2, 2004·Biological & Pharmaceutical Bulletin·Takahiro WatanabeMisao Kubo
Apr 14, 2005·Journal of Agricultural and Food Chemistry·Isabel TaverniersMarc De Loose

❮ Previous
Next ❯

Citations

Jan 27, 2010·BMC Evolutionary Biology·Paulina SchmittJulien de Lorgeril
Jun 10, 2008·Analytical and Bioanalytical Chemistry·Elisa MicheliniAldo Roda
Sep 3, 2013·Biological & Pharmaceutical Bulletin·Osamu NakajimaReiko Teshima
Oct 16, 2015·Analytical and Bioanalytical Chemistry·Jun LiGang Wu
Jun 15, 2011·Food Chemistry·Rafaat M ElsanhotyKlaus Dieter Jany
Aug 2, 2013·Applied Biochemistry and Biotechnology·Rafaat M ElsanhotyMohamed Fawzy Ramadan
May 23, 2008·Journal of Agricultural and Food Chemistry·Sergio SalviGiorgio Morelli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.