PMID: 9647822Jul 2, 1998Paper

Quantification of Gordona amarae strains in foaming activated sludge and anaerobic digester systems with oligonucleotide hybridization probes

Applied and Environmental Microbiology
M F de los ReyesL Raskin

Abstract

Previous studies have shown the predominance of mycolic acid-containing filamentous actinomycetes (mycolata) in foam layers in activated sludge systems. Gordona (formerly Nocardia) amarae often is considered the major representative of this group in activated sludge foam. In this study, small-subunit rRNA genes of four G. amarae strains were sequenced, and the resulting sequences were compared to the sequence of G. amarae type strain SE-6. Comparative sequence analysis showed that the five strains used represent two lines of evolutionary descent; group 1 consists of strains NM23 and ASAC1, and group 2 contains strains SE-6, SE-102, and ASF3. The following three oligonucleotide probes were designed: a species-specific probe for G. amarae, a probe specific for group 1, and a probe targeting group 2. The probes were characterized by dissociation temperature and specificity studies, and the species-specific probe was evaluated for use in fluorescent in situ hybridizations. By using the group-specific probes, it was possible to place additional G. amarae isolates in their respective groups. The probes were used along with previously designed probes in membrane hybridizations to determine the abundance of G. amarae, group 1, group 2,...Continue Reading

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Citations

Oct 25, 2006·Water Environment Research : a Research Publication of the Water Environment Federation·Domènec Jolis, Matina Marneri
May 1, 2007·FEMS Microbiology Ecology·Caroline KragelundPer Halkjaer Nielsen
Jun 11, 2009·Environmental Technology·Matina MarneriEfi Koutsiouki
Feb 18, 2005·Letters in Applied Microbiology·A KaetzkeK Eschrich
Jun 9, 2004·Applied and Environmental Microbiology·Matthias ArenskötterAlexander Steinbüchel
Jul 15, 2011·Water Environment Research : a Research Publication of the Water Environment Federation·Dashika NaidooFaizal Bux
Jul 4, 2003·Applied and Environmental Microbiology·Gomathinayagam PonniahPaul Blum
Aug 26, 2017·Scientific Reports·Rasmus H KirkegaardPer H Nielsen
Oct 20, 2005·Journal of Bioscience and Bioengineering·K Watanabe, P W Baker
Sep 19, 2000·Journal of Microbiological Methods·A Moter, U B Göbel
May 2, 2000·Applied and Environmental Microbiology·D B OertherL Raskin
Jul 28, 2021·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Jiří Wanner

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