PMID: 7545150Sep 1, 1995Paper

Quantification of group A colicin import sites

Journal of Bacteriology
Denis DuchéD Baty

Abstract

Pore-forming colicins are soluble bacteriocins which form voltage-gated ion channels in the inner membrane of Escherichia coli. To reach their target, these colicins first bind to a receptor located on the outer membrane and then are translocated through the envelope. Colicins are subdivided into two groups according to the envelope proteins involved in their translocation: group A colicins use the Tol proteins; group B colicins use the proteins TonB, ExbB, and ExbD. We have previously shown that a double-cysteine colicin A mutant which possesses a disulfide bond in its pore-forming domain is translocated through the envelope but is unable to form a channel in the inner membrane (D. Duché, D. Baty, M. Chartier, and L. Letellier, J. Biol. Chem. 269:24820-24825, 1994). Measurements of colicin-induced K+ efflux reveal that preincubation of the cells with the double-cysteine mutant prevents binding of colicins of group A but not of group B. Moreover, we show that the mutant is still in contact with its receptor and import machinery when it interacts with the inner membrane. From these competition experiments, we conclude that each Escherichia coli cell contains approximately 400 and 1,000 colicin A receptors and translocation sites...Continue Reading

References

Apr 5, 1992·Journal of Molecular Biology·M W ParkerD Tsernoglou
Apr 1, 1990·Molecular Microbiology·W A CramerH Y Song
Oct 1, 1990·Molecular Microbiology·J P BourdineaudJ M Pagès
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J P BourdineaudL Letellier
Jan 5, 1989·Nature·M W ParkerD Tsernoglou
Nov 1, 1988·Molecular Microbiology·D BatyC Lazdunski
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Sep 14, 1993·Biochemistry·S F MelR M Stroud

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Citations

Oct 7, 2010·The Journal of Biological Chemistry·Xiang Y-Z ZhangDenis Duché
Jun 28, 1996·The Journal of Biological Chemistry·D DuchéD Baty
Mar 9, 1999·Folia Microbiologica·J Smarda, D Smajs
Feb 3, 1999·Annual Review of Genetics·M A Riley
Nov 28, 2012·Biochemical Society Transactions·Miklos de Zamaroczy, Liliana Mora
Jan 30, 1997·Nature·M WienerR M Stroud
Feb 11, 1998·Journal of Bacteriology·P ChamesD Duché
Oct 3, 2006·Journal of Bacteriology·Denis DuchéRoland Lloubès
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Sep 28, 1998·Journal of Bacteriology·C J LazdunskiH Bénédetti
Sep 8, 1998·Current Opinion in Structural Biology·R M StroudD Freymann
Nov 25, 1998·Research in Microbiology·R el KouhenJ M Pagès
Sep 1, 1996·Journal of Bacteriology·R El Kouhen, J M Pagès

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