Quantification of hepatic transaldolase exchange activity and its effects on tracer measurements of indirect pathway flux in humans

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
John G JonesLuisa Diogo

Abstract

Exchange of hepatic glucose-6-phosphate (G6P) and glyceraldehyde-3-phosphate via transaldolase modifies hepatic G6P enrichment from glucose or gluconeogenic tracers. Transaldolase exchange was quantified in five healthy, fed subjects following an oral bolus of [1,2,3-(13)C(3)]glycerol (25-30 mg/kg) and paracetamol (10-12 mg/kg). (13)C Isotopomers of hepatic G6P were quantified by (13)C NMR spectroscopy of urinary glucuronide. [1,2,3-(13)C(3)]- and [4,5,6-(13)C(3)]glucuronide isotopomers, representing the conversion of [1,2,3-(13)C(3)]glycerol to G6P via dihydroxyacetone phosphate, were resolved from [1,2-(13)C(2)]- and [5,6-(13)C(2)]glucuronide (13)C-isotopomers, derived from metabolism of [1,2,3-(13)C(3)]glycerol via pyruvate and phosphoenolpyruvate. Enrichment of [1,2,3-(13)C(3)]glucuronide was significantly less than that of [4,5,6-(13)C(3)]glucuronide (1.30 +/- 0.57% versus 1.67 +/- 0.42%, P < 0.05). Also, [1,2-(13)C(2)]glucuronide enrichment was significantly less than that of [5,6-(13)C(2)]glucuronide (0.28 +/- 0.08% versus 0.36 +/- 0.03%, P < 0.05). Transaldolase and triose phosphate isomerase exchange activities were estimated by applying the (13)C-isotopomer data to a model of hepatic sugar phosphate metabolism. Triose...Continue Reading

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Citations

Dec 14, 2012·The Journal of Biological Chemistry·Eunsook S JinCraig R Malloy
Oct 4, 2012·American Journal of Physiology. Endocrinology and Metabolism·Jeffrey D Browning, Shawn C Burgess
Nov 11, 2010·American Journal of Physiology. Endocrinology and Metabolism·Rita BasuRobert Rizza
Aug 25, 2011·Metabolism: Clinical and Experimental·Cristina BarosaJohn G Jones
Sep 26, 2009·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Teresa C DelgadoJohn G Jones
May 30, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Patricia M NunesJohn G Jones
May 23, 2015·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Margarida CoelhoJohn G Jones
Oct 8, 2014·The Journal of Biological Chemistry·Eunsook S JinCraig R Malloy
Jun 6, 2013·American Journal of Physiology. Endocrinology and Metabolism·Aman RajpalRita Basu

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