Quantification of Histamine and Carcinine in Drosophila melanogaster Tissues

ACS Chemical Neuroscience
Madelaine E DennoB Jill Venton

Abstract

Histamine is a neurotransmitter crucial to the visual processing of Drosophila melanogaster. It is inactivated by metabolism to carcinine, a β-alanyl derivative, and the same enzyme that controls that process also converts dopamine to N-β-alanyl-dopamine. Direct detection of histamine and carcinine has not been reported in single Drosophila brains. Here, we quantify histamine, carcinine, dopamine, and N-β-alanyl-dopamine in Drosophila tissues by capillary electrophoresis coupled to fast-scan cyclic voltammetry (CE-FSCV). Limits of detection were low, 4 ± 1 pg for histamine, 10 ± 4 pg for carcinine, 2.8 ± 0.3 pg for dopamine, and 9 ± 3 pg for N-β-alanyl-dopamine. Tissue content was compared in the brain, eyes, and cuticle from wild-type (Canton S) and mutant (tan(3) and ebony(1)) strains. In tan(3) mutants, the enzyme that produces histamine from carcinine is nonfunctional, whereas in ebony(1) mutants, the enzyme that produces carcinine from histamine is nonfunctional. In all fly strains, the neurotransmitter content was highest in the eyes and there were no strain differences for tissue content in the cuticle. The main finding was that carcinine levels changed significantly in the mutant flies, whereas histamine levels did not....Continue Reading

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Dec 2, 2014·ACS Chemical Neuroscience·Madelaine E DennoB Jill Venton
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Citations

Jun 7, 2019·Addiction Biology·Rebecca E SchmittMike Grotewiel
Aug 11, 2018·Frontiers in Molecular Biosciences·Brian G O'FlynnDavid J Merkler
Feb 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Thierry IzoréMax J Cryle
Mar 28, 2019·Analytical Methods : Advancing Methods and Applications·Dilpreet RajuAlexander G Zestos
Jun 14, 2019·Analytical Chemistry·Pumidech PuthongkhamB Jill Venton
Jan 21, 2017·Analytical Chemistry·Mallikarjunarao GanesanaB Jill Venton
Mar 10, 2018·Analytical Chemistry·Marissa R CabayScott A Shippy
Feb 8, 2018·ACS Chemical Neuroscience·Mimi ShinB Jill Venton

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