Quantification of HIV-1 proviral DNA by a standardized colorimetric PCR-based assay

Journal of Medical Virology
J IzopetJ Puel

Abstract

A simple method was developed for measuring human immunodeficiency virus type 1 (HIV-1) proviral DNA in mononuclear cells based on the commercially available Amplicor(TM) HIV-1 polymerase chain reaction (PCR) assay and the limiting dilution method. The lowest limit of detection was four proviral genomes per 10(6) cells. The accuracy was demonstrated by using serial dilutions of LAV-8E5 cells, and the interassay variability was 0.2 log. The technique was used to measure HIV-1 proviral DNA in the peripheral blood mononuclear cells (PBMC) of 18 antiretroviral drug-naive HIV-1-positive individuals before and 4 weeks after initiating double nucleoside therapy. The DNA proviral titers at baseline (median = 3.45, range = 2.11-4.7 log copies/10(6) cells) were 2.08 log greater than the infectivity titers, but there was a correlation between these two parameters (r = 0.63, P = 0.009). The mean decrease in the proviral DNA titer after 4 weeks of therapy was 0.31 log, whereas the decrease in the infectivity titer was 0.81 log and the decrease in the plasma RNA concentration was 1.29 log. The technique was also used to measure HIV-1 proviral DNA in the PBMC of 11 patients who had undetectable plasma HIV-1 RNA after being placed on combinati...Continue Reading

References

Jul 1, 1995·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·A KhadirA Kessous-Elbaz
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Citations

Apr 3, 2001·Journal of Clinical Microbiology·N DésiréJ C Nicolas
Feb 5, 2002·Journal of Clinical Microbiology·Yuqi ZhaoRam Yogev
Dec 16, 2005·Cell Research·Niel T ConstantineRichard Y Zhao
Jul 14, 2006·The European Journal of Neuroscience·Takashi TorashimaHirokazu Hirai
May 25, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Francesca VitoneMaria Carla Re
Oct 3, 1999·AIDS Research and Human Retroviruses·S AlemanA Sönnerborg
Aug 26, 2016·Clinical Microbiology Reviews·Véronique Avettand-FènoëlChristine Rouzioux
Dec 22, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·J IzopetJ Puel
Feb 15, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·A LafeuilladeE Delbeke

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