Quantification of HIV-1 viral load in lymphoid and blood cells: assessment during four-drug combination therapy

AIDS
C TamaletN Yahi

Abstract

To assess the antiretroviral effect of a combination of zidovudine, didanosine, lamivudine and saquinavir in plasma, peripheral blood mononuclear cells (PBMC) and lymph-node mononuclear cells (LNMC) after 8 weeks. Ten HIV-1 antiretroviral therapy-naive patients were given a combination of oral zidovudine (200 mg three times daily), oral didanosine (200 twice a day), oral lamivudine (150 mg twice a day) and oral saquinavir (600 mg three times daily). HIV-1 plasma RNA was measured by quantitative reverse transcriptase (RT)-polymerase chain reaction (PCR). Infectious HIV-1 in PBMC and LNMC was measured by a coculture technique. HIV-1 RNA in PBMC and LNMC was quantified by RT-PCR. Proviral DNA titres in PBMC and LNMC were measured by endpoint dilution PCR. CD4 T-cells were analysed by flow cytometry. CD4 cell counts rose in all patients (mean increase of 125 +/- 71 CD4 cells x 10(6)/l) and the benefit was greater for patients with fewer than 350 CD4 cells x 10(6)/l (mean increase of 159 +/- 74 CD4 cells x 10(6)/l). Plasma HIV-1 RNA decreased exponentially in all patients (mean decrease of 3.1 log10 after 8 weeks with a mean half-life of 2.2 +/- 0.6 days). HIV-1 RNA showed a decrease of 3.07 log10 in PBMC and of 2.1 log10 in LNMC. T...Continue Reading

References

Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·O J CohenR T Steigbigel
Aug 17, 1995·The New England Journal of Medicine·D D Ho
Aug 1, 1996·The Journal of Infectious Diseases·A LafeuilladeO Costes

❮ Previous
Next ❯

Citations

Feb 24, 1999·AIDS Research and Human Retroviruses·S ZanussiP De Paoli
Apr 30, 2002·Current Opinion in Infectious Diseases·H J Stellbrink, J van Lunzen
May 19, 2017·The Journal of Infectious Diseases·Eli A Boritz, Daniel C Douek
Mar 19, 1999·AIDS Research and Human Retroviruses·M VaillancourtR Swanstrom
Oct 3, 1999·AIDS Research and Human Retroviruses·S AlemanA Sönnerborg
Jun 24, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·S ZanussiP De Paoli
Apr 1, 2000·AIDS Patient Care and STDs·A K Iversen
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·N M FergusonR M Anderson
Aug 1, 1997·Expert Opinion on Investigational Drugs·S Morris-JonesP J Easterbrook
Apr 8, 2003·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Joy M FolkvordElizabeth Connick
Oct 30, 2014·AIDS Research and Human Retroviruses·Michelle K HaasElizabeth Connick
Dec 22, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·J IzopetJ Puel
Feb 6, 1999·The Journal of the Association of Nurses in AIDS Care : JANAC·N R ReynoldsD E Brashers
Apr 3, 2001·Journal of Clinical Microbiology·N DésiréJ C Nicolas
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Oct 4, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Saleta SierraRolf Kaiser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.