Quantification of homocysteine and cysteine by derivatization with pyridoxal 5'-phosphate and hydrophilic interaction liquid chromatography

Analytical and Bioanalytical Chemistry
R GłowackiHieronim Jakubowski

Abstract

A simple and rapid assay using pyridoxal 5'-phosphate (PLP) as a derivatizing reagent was developed for the simultaneous determination of homocysteine (Hcy) and cysteine (Cys) in human plasma. Derivatization with PLP affords UV-absorbing tetrahydrothiazine and thiazolidine derivatives of Hcy and Cys, respectively. Separation of these derivatives was achieved in 5 min using a hydrophilic interaction liquid chromatography, followed by UV detection at 330 nm. Linearity in detector response was observed over the range of 0.25-20 μM for Hcy and 10-300 μM for Cys. The limit of quantification (LOQ) values for Hcy and Cys were 0.25 and 2.5 μM, respectively. The method was successfully applied to plasma samples donated by apparently healthy volunteers.

References

Aug 19, 1992·JAMA : the Journal of the American Medical Association·M J StampferC H Hennekens
Feb 1, 1996·Journal of Pharmaceutical and Biomedical Analysis·S BraggioM Cugola
Sep 3, 1999·Clinical and Diagnostic Laboratory Immunology·B RojkovichP Gergely
May 16, 2000·Biochemical Pharmacology·W A Kleinman, J P Richie
May 15, 2001·Clinical Chemistry and Laboratory Medicine : CCLM·A AnderssonB Hultberg
Feb 19, 2002·Kidney International·Jonathan HimmelfarbEllen McMonagle
Mar 5, 2004·Cellular and Molecular Life Sciences : CMLS·H Jakubowski
Sep 5, 2006·Chemistry : a European Journal·Hieronim Jakubowski
Apr 10, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Toshimasa Toyo'oka
Apr 24, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Krzysztof KuśmierekEdward Bald
Jun 30, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rafał Głowacki, Edward Bald
Nov 30, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·K KuśmierekE Bald
Oct 8, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C L GopuK Sreenivasan
Dec 2, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhao-Yun CaoMing-Xue Chen
Jan 24, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kamila BorowczykRafał Głowacki

❮ Previous
Next ❯

Citations

Jul 22, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yao SunJiang Zheng
Jun 16, 2017·Molecular Carcinogenesis·Renu PandeyStefano Tiziani
Feb 13, 2021·Cardiovascular Research·Magnus Bäck, Jean-Baptiste Michel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.