Quantification of human adenovirus and norovirus in river water in the north-east of France

Environmental Science and Pollution Research International
Maryse Iris SedjiIsabelle Bertrand

Abstract

Human adenoviruses (HAdVs) are a major cause of infection and have been proposed as viral indicators of water quality. Human noroviruses (NoV) are the main cause of viral acute gastroenteritis. Quantitative data on the environmental prevalence of both viruses are needed. The genomes of HAdVs enteric adenovirus type 41 (HAdV41) and noroviruses of genogroups I and II (NoV GGI and GGII) were quantified over a 6-month period in a river located in north-eastern France. The samples were collected downstream from the discharge of a wastewater treatment plant. The viruses were concentrated using a glass wool method and the viral genomes were quantified using digital droplet PCR (ddPCR). All river water samples (15/15) were positive for the genomes of HAdVs, HAdV41, NoV GGI and NoV GGII. Concentrations of HAdVs, HAdV41 and NoV GII genomes were similar and HAdV41 represented ~ 80% of HAdVs. Infectious HAdVs were quantified in these samples using an integrated cell culture-quantitative PCR method (ICC-qPCR); they were detected in 93% (14/15) and quantified in 53% (8/15) of the samples. Thus, infectious HAdVs represented 0.3 to 12.2% of total HAdV particles detected by ddPCR. Infectious HAdV41 particles were found in 73% (11/15) of the sam...Continue Reading

References

Oct 24, 2002·Journal of Applied Microbiology·G E GreeningG D Lewis
Jul 4, 2003·Applied and Environmental Microbiology·Benoît GassilloudChristophe Gantzer
Dec 9, 2003·Applied and Environmental Microbiology·Gwangpyo KoMark D Sobsey
Oct 10, 2006·Applied and Environmental Microbiology·Silvia Bofill-MasRosina Girones
Oct 16, 2007·Applied and Environmental Microbiology·Irene XagorarakiJoan B Rose
Nov 30, 2007·Journal of Applied Microbiology·E HaramotoS Ohgaki
Nov 7, 2008·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Daniel GerrityMorteza Abbaszadegan
Oct 13, 2009·Memórias do Instituto Oswaldo Cruz·Joselma Siqueira-SilvaCharlotte Marianna Hársi
Jul 14, 2010·Water Research·Rosina GironesSílvia Bofill-Mas
Oct 19, 2010·Applied and Environmental Microbiology·Leslie OgorzalyChristophe Gantzer
Nov 26, 2010·Water Research·A Peter Wyn-JonesMark Wyer
Nov 19, 2011·FEMS Microbiology Reviews·David Rodríguez-LázaroWim H M van der Poel
Mar 3, 2012·Journal of Applied Microbiology·I BertrandC Gantzer
Apr 6, 2013·Applied Microbiology and Biotechnology·Leslie OgorzalyIsabelle Bertrand
Apr 29, 2015·Food and Environmental Virology·Leslie OgorzalyHenry-Michel Cauchie
Jun 2, 2015·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Hodon RyuSamuel Hayes
Aug 25, 2015·Environmental Science and Pollution Research International·Gislaine FongaroC R M Barardi
Dec 30, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K KosulinT Lion
Jan 20, 2016·Food and Environmental Virology·Carmen Baur VieiraMarize Pereira Miagostovich
Apr 12, 2016·Water Research·Emily RamesJoanne Macdonald
Sep 4, 2016·International Journal of Hygiene and Environmental Health·Christian TimmThomas Kistemann
Nov 27, 2016·Food and Environmental Virology·Adrien BriéIsabelle Bertrand
Jun 10, 2017·Environmental Science & Technology·Charles P Gerba, Walter Q Betancourt
Jun 24, 2017·Environmental Science and Pollution Research International·Yi-Jia ShihBing-Mu Hsu
Feb 7, 2018·Environmental Science and Pollution Research International·Chourouk IbrahimSalah Hammami
May 2, 2018·The Science of the Total Environment·Kata FarkasDavey L Jones

❮ Previous
Next ❯

Citations

Jun 28, 2019·Frontiers in Microbiology·Francisco Martinez-HernandezManuel Martinez-Garcia
Sep 10, 2020·Chemical Engineering Journal·S Venkata MohanA Kiran Kumar
Mar 2, 2021·Environmental Chemistry Letters·Simranjeet SinghJoginder Singh
Apr 22, 2021·ACS ES&T Water·Jamie D ShutlerRavinder S Dahiya
Aug 21, 2021·Journal of Hazardous Materials·Kaige SongFei Ling

❮ Previous
Next ❯

Related Concepts