Quantification of immunosuppression by flow cytometric measurement of intracellular cytokine synthesis

Transplant International : Official Journal of the European Society for Organ Transplantation
A P van den BergL F de Leij

Abstract

The availability of a method to measure the effects of drugs on immune reactivity should be helpful in optimizing treatment after organ transplantation. Since cyclosporine A (CSA) interferes with activation of T cells and cytokine synthesis, production of IL-2 and IFN-gamma might constitute a marker of this drug's effects. We measured the capacity for mitogen-stimulated production of these cytokines in whole blood by using immunostaining of intracellular and membrane antigens, followed by flow cytometry. The percentage of CD4+ T cells producing IL-2 or IFN-gamma was strongly reduced in 20 transplant patients compared with 24 healthy controls. The capacity for IL-2 production of CD4+ and CD8+ cells correlated inversely with CSA blood levels (P values 0.0087 and 0.0396, respectively). IFN-gamma production by CD4+ T cells showed a negative correlation with the prednisolone dose (P = 0.0175) and, for the CD8+ subset, with CSA trough levels (P = 0.0023). These data show that inhibition of T cell cytokine synthesis by CSA and prednisolone can be quantified.

Citations

Mar 27, 2001·Transplantation Proceedings·A P van den BergL M de Leij
Dec 21, 2002·Transplantation Proceedings·M NowaczykG Korczak-Kowalska
Sep 2, 1998·Transplantation Proceedings·A P van den BergL H de Leij
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Dec 10, 1999·The Journal of Clinical Investigation·W L TrepicchioJ G Krueger
Sep 16, 2004·Intensive Care Medicine·Thierry FumeauxJérôme Pugin
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Nov 22, 2005·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Ingo KaczmarekBruno Reichart
Jan 14, 2011·Veterinary Immunology and Immunopathology·C L FellmanA J Mackin
Nov 25, 2011·PLoS Pathogens·Grégoire ChevalierDaniel Gonzalez-Dunia
Mar 17, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Jochen KluppRandall E Morris

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