Quantification of ion transport in perfused rat heart: 133Cs+ as an NMR active K+ analog

The American Journal of Physiology
P A SchornackJ J Ackerman

Abstract

Proper ion balance between intra- and extracellular compartments is necessary for normal physiological function. Conversely, alterations in membrane ion transport occur in numerous pathological states. As a noninvasive, nondestructive spectroscopic technique, nuclear magnetic resonance (NMR) offers a powerful approach to the study of ion balance in intact biological systems. Unfortunately, rare NMR active nuclides that are isotopes of the 100% naturally abundant 23Na+ and 39K+ are not available for tracer kinetic studies of Na1 and K+ transport. However, Cs is a biologically active analog of K+, and the 100% naturally abundant NMR active 133Cs+ nuclide can be employed to examine K+ transport (Davis, D. G., E. Murphy, and R. E. London. Biochemistry 27: 3547-3551, 1988). The distinguishing feature of 133Cs+ is that it naturally gives two separate well-resolved NMR resonances for intra- and extra-cellular 133Cs+, permitting study of the time course changes of either of these compartments independent of the other. In this report, the experimental procedures and compartmental modeling formalism are developed that allow quantitative analysis of Cs+ membrane transport in the perfused rat heart. Intracellular 133Cs+ is shown to be 100%...Continue Reading

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Citations

Apr 3, 2001·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J M ColetR N Muller
Nov 4, 2005·Acta Physiologica Scandinavica·M Kaikkonen, S Hyyppä
Mar 17, 2005·NMR in Biomedicine·James GoodmanJoseph J H Ackerman
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Jun 7, 2017·Chemical Communications : Chem Comm·M KarlssonM H Lerche
Jan 11, 2002·Journal of Neurophysiology·Raimondo D'AmbrosioH Richard Winn
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Jan 24, 1998·Biochemical and Biophysical Research Communications·M L GruwelJ Schrader

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