Quantification of Listeria monocytogenes in minimally processed leafy vegetables using a combined method based on enrichment and 16S rRNA real-time PCR

Food Microbiology
Maria Aparecida de OliveiraElaine Cristina Pereira De Martinis

Abstract

Modern lifestyle markedly changed eating habits worldwide, with an increasing demand for ready-to-eat foods, such as minimally processed fruits and leafy greens. Packaging and storage conditions of those products may favor the growth of psychrotrophic bacteria, including the pathogen Listeria monocytogenes. In this work, minimally processed leafy vegetables samples (n = 162) from retail market from Ribeirão Preto, São Paulo, Brazil, were tested for the presence or absence of Listeria spp. by the immunoassay Listeria Rapid Test, Oxoid. Two L. monocytogenes positive and six artificially contaminated samples of minimally processed leafy vegetables were evaluated by the Most Probable Number (MPN) with detection by classical culture method and also culture method combined with real-time PCR (RTi-PCR) for 16S rRNA genes of L. monocytogenes. Positive MPN enrichment tubes were analyzed by RTi-PCR with primers specific for L. monocytogenes using the commercial preparation ABSOLUTE QPCR SYBR Green Mix (ABgene, UK). Real-time PCR assay presented good exclusivity and inclusivity results and no statistical significant difference was found in comparison with the conventional culture method (p < 0.05). Moreover, RTi-PCR was fast and easy to p...Continue Reading

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Citations

Mar 16, 2013·Foodborne Pathogens and Disease·Bruna Carrer GomesElaine Cristina Pereira De Martinis
Nov 16, 2010·Applied and Environmental Microbiology·Evelyn M ClaytonR Paul Ross
May 17, 2011·Food Microbiology·Florence PostollecDanièle Sohier
Jan 25, 2011·Journal of Microbiological Methods·Urban TraunšekMinka Kovač
Dec 19, 2015·Food Microbiology·Anais Auvolat, Nathalie Gnanou Besse
Nov 19, 2015·Frontiers in Microbiology·Jodi Woan-Fei LawLearn-Han Lee
Mar 20, 2012·Research in Microbiology·Luisa Zanolli MorenoAndrea Micke Moreno
Jun 11, 2020·Critical Reviews in Food Science and Nutrition·Maiara da Costa Lima, Evandro Leite de Souza
Nov 14, 2019·Canadian Journal of Microbiology·Kristine Castañeda-GullaAnthony N Mutukumira

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