Quantification of low molecular weight selenium metabolites in human plasma after treatment with selenite in pharmacological doses by LC-ICP-MS

Analytical and Bioanalytical Chemistry
Konstantina FloudaBente Gammelgaard

Abstract

The paper presents an analytical method for quantification of low molecular weight (LMW) selenium compounds in human plasma based on liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS) and post column isotope dilution-based quantification. Prior to analysis, samples were ultrafiltrated using a cut-off value of 3000 Da. The method was validated in aqueous solution as well as plasma using standards of selenomethionine (SeMet), Se-methylselenocysteine (MeSeCys), selenite, and the selenosugar Se-methylseleno-N-acetylgalactosamine (SeGal) for linearity, precision, recoveries, and limits of detection and quantitation with satisfactory results. The method was applied for analysis of a set of plasma samples from cancer patients receiving selenite treatment in a clinical trial. Three LMW selenium compounds were observed. The main compounds, SeGal and selenite were tentatively identified by retention time matching with standards in different chromatographic systems, while the third minor compound was not identified. The identity of the selenosugar was verified by ESI-MS-MS product ion scanning, while selenite was identified indirectly as the glutathione (GSH) reaction product, GS-Se-SG.

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Citations

Feb 12, 2017·Metallomics : Integrated Biometal Science·T A MarschallT Schwerdtle
Oct 30, 2019·Metallomics : Integrated Biometal Science·Barbara R CardosoBlaine R Roberts
Nov 4, 2020·Analytical and Bioanalytical Chemistry·Christian L Ward-DeitrichHeidi Goenaga-Infante
Jul 3, 2021·Antioxidants·Tabitha Jenkins, Jerome Gouge

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