Quantification of major lipid classes in human triacylglycerol-rich lipoproteins by high-performance liquid chromatography with evaporative light-scattering detection

Journal of Separation Science
Javier S Perona, Valentina Ruiz-Gutierrez

Abstract

Triacylglycerol-rich lipoproteins (TRL), comprising chylomicrons (CM) and very-low-density lipoproteins (VLDL), have been associated with cardiovascular disease. The lipid class content in the remnant particles of these lipoproteins is a determinant for the accumulation of lipids in macrophages and their transformation into foam cells. We have optimized a method for the simultaneous determination of cholesteryl esters (CE), triacylglycerols (TG), free cholesterol (FC), monoacylglycerols (MG), and phospholipids (PL) by HPLC coupled to a light-scattering detector (ELSD). A diol column and a ternary gradient of hexane, 2-propanol, and methanol were applied to CM and VLDL of human origin (n = 10), with excellent precision in terms of repeatability of peak areas and retention times. All peaks were baseline resolved although the resolution of CE and TG was compromised for the sake of simplicity of the solvent gradient. The ELSD response was fitted to second-order equations, with correlation coefficients (r2) higher than 0.999 for a wide range of concentrations (0.25-10 microg of lipid injected). TG were the major lipid class detected in human TRL, accounting for 62% in CM obtained 2 h after the oil intake. In addition we recorded a d...Continue Reading

References

Jan 3, 1992·Biochimica Et Biophysica Acta·C S WangW J McConathy
Feb 1, 1985·Biochemical Society Transactions·H Jansen, W C Hülsmann
Nov 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J H RappJ P Kane
Mar 1, 1994·Atherosclerosis·F KarpeA Hamsten
May 9, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·N U Olsson, N Salem
Feb 3, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·C Silversand, C Haux
Apr 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J S PeronaV Ruiz-Gutiérrez
Oct 26, 2000·Current Opinion in Lipidology·M SakaiS Horiuchi
May 8, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·T Seppänen-LaaksoR Hiltunen
Jan 25, 2002·Annals of the New York Academy of Sciences·A KawakamiF Numano

❮ Previous
Next ❯

Citations

Oct 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sumana Raychaudhuri, William A Prinz
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cindy Y JaoAdrian Salic
Oct 17, 2007·The Journal of General Physiology·Mirela MilescuKenton J Swartz
Aug 1, 2008·Molecular Biology of the Cell·Christine M FederovitchRandolph Y Hampton
Nov 22, 2007·Virology Journal·Joshua M CostinWilliam C Wimley
Nov 8, 2011·Journal of Lipid Research·Bernard P C KokDavid N Brindley
Jul 2, 2011·Biological & Pharmaceutical Bulletin·Runmei YangNannan Gao
Jan 13, 2009·Journal of Biomolecular Techniques : JBT·Jan WillmannHerbert Thiele
Sep 24, 2014·The British Journal of Nutrition·Rosana Cabello-MorunoJavier S Perona
Dec 8, 2009·The Journal of Nutritional Biochemistry·Javier S PeronaValentina Ruiz-Gutierrez
Oct 12, 2013·The Journal of Membrane Biology·Javier S PeronaFrancisca Barceló
Mar 16, 2011·Analytical Biochemistry·David G McLarenThomas P Roddy
Oct 27, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Hicham BerrouguiAbdelouahed Khalil
Jan 19, 2013·Journal of Liposome Research·Aranzazu Antelo, Javier S Perona
Aug 25, 2016·Journal of Chromatography. a·Nolwenn PrachePierre Chaminade
May 26, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Laura Rabinovich-GuilattPierre Chaminade
Feb 12, 2017·Journal of the Science of Food and Agriculture·Barbara KusznierewiczAgnieszka Bartoszek
Oct 18, 2011·Journal of Separation Science·Christian HellmuthBerthold Koletzko
Nov 2, 2014·Journal of Separation Science·Václav Matěj BierhanzlPeter Podolec
Sep 14, 2007·Molecular Membrane Biology·Jesús PradesFrancisca Barceló
Mar 14, 2007·Journal of the American College of Nutrition·Rosana Cabello-MorunoValentina Ruiz-Gutierrez
May 22, 2007·Biochemical Society Transactions·C BentleyC Lawson
Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dariana G Rodríguez-SánchezCarmen Hernández-Brenes
Nov 28, 2017·Translational Research : the Journal of Laboratory and Clinical Medicine·Ahmad F HedayatLilach O Lerman

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.