Quantification of Methylphenidate, Dexamphetamine, and Atomoxetine in Human Serum and Oral Fluid by HPLC With Fluorescence Detection

Therapeutic Drug Monitoring
Benedikt StegmannEkkehard Haen

Abstract

For psychostimulants, a marked individual variability in the dose-response relationship and large differences in plasma concentrations after similar doses are known. Therefore, optimizing the efficacy of these drugs is at present the most promising way to exploit their full pharmacological potential. Moreover, it seems important to examine oral fluid as less invasive biological matrix for its benefit in therapeutic drug monitoring for patients with hyperkinetic disorder. A high-performance liquid chromatography method for quantification of methylphenidate (MPH), dexamphetamine (DXA), and atomoxetine in serum and oral fluid has been developed and validated. The analytical procedure involves liquid-liquid extraction, derivatization with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride as a label and chromatographic separation on a Phenomenex Gemini-NX C18 analytical column using gradient elution with water-acetonitrile. The derivatized analytes were detected at 330 nm (excitation wavelength) and 440 nm (emission wavelength). To examine the oral fluid/serum ratios, oral fluid samples were collected simultaneously to blood samples from patients with hyperkinetic disorder. The method allows quantification of all analytes in serum a...Continue Reading

References

Nov 1, 1992·Clinical Pharmacokinetics·R K Drobitch, C K Svensson
Jan 11, 2000·Clinical Pharmacokinetics·H C KimkoD R Abernethy
Jun 14, 2003·Journal of Child and Adolescent Psychopharmacology·Jennifer W WitcherJoseph Biederman
Aug 1, 1956·Journal of Cellular Physiology·A S BURGEN
Apr 15, 2004·The Journal of Pharmacology and Experimental Therapeutics·Zejin SunWilliam F Bosron
May 25, 2005·Clinical Pharmacokinetics·John-Michael SauerJennifer W Witcher
Nov 22, 2005·Expert Opinion on Drug Delivery·Kennerly S PatrickJohn S Markowitz
Sep 7, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hao-Jie ZhuJohn S Markowitz
Dec 30, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ralf KoeberEkkehard Haen
Feb 22, 2013·Zeitschrift für Kinder- und Jugendpsychiatrie und Psychotherapie·Benedikt StegmannEkkehard Haen
May 9, 2015·Zeitschrift für Kinder- und Jugendpsychiatrie und Psychotherapie·Benedikt StegmannEkkehard Haen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.