Quantification of nanoparticle endocytosis based on double fluorescent pH-sensitive nanoparticles

Biomedical Microdevices
Andréa Kurtz-ChalotValérie Forest

Abstract

Amorphous silica is a particularly interesting material because of its inertness and chemical stability. Silica nanoparticles have been recently developed for biomedical purposes but their innocuousness must be carefully investigated before clinical use. The relationship between nanoparticles physicochemical features, their uptake by cells and their biological activity represents a crucial issue, especially for the development of nanomedicine. This work aimed at adapting a method for the quantification of nanoparticle endocytosis based on pH-sensitive and double fluorescent particles. For that purpose, silica nanoparticles containing two fluorophores: FITC and pHrodo(TM) were developed, their respective fluorescence emission depends on the external pH. Indeed, FITC emits a green fluorescence at physiological pH and pHrodo(TM) emits a red fluorescence which intensity increased with acidification. Therefore, nanoparticles remained outside the cells could be clearly distinguished from nanoparticles uptaken by cells as these latter could be spotted inside cellular acidic compartments (such as phagolysosomes, micropinosomes…). Using this model, the endocytosis of 60 nm nanoparticles incubated with the RAW 264.7 macrophages was quant...Continue Reading

References

Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S Ohkuma, B Poole
Mar 6, 2002·Archives of Toxicology·R MergetT Bruening
Jul 13, 2004·Trends in Cell Biology·Gareth Griffiths
Aug 28, 2004·International Journal of Hygiene and Environmental Health·Joachim BruchBice Fubini
Jan 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Indrajit RoyParas N Prasad
Apr 13, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jari Nuutila, Esa-Matti Lilius
Jul 9, 2005·Environmental Health Perspectives·Günter OberdörsterJan Oberdörster
Dec 17, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katrina M WatersBrian D Thrall
Jan 15, 2009·Angewandte Chemie·Kristina RiehemannHarald Fuchs
Nov 17, 2009·Journal of Nanoscience and Nanotechnology·J FizetO Tillement
Apr 10, 2010·Chemical Communications : Chem Comm·Eiji NakataHitoshi Hori
Feb 4, 2011·Angewandte Chemie·Wendelin J Stark
Jun 15, 2011·The Journal of Clinical Investigation·Miriam BenezraMichelle S Bradbury
Nov 8, 2011·Methods in Molecular Biology·Venkat K Ramshesh, John J Lemasters
Jan 24, 2012·Methods in Enzymology·Yong-Eun Koo Lee, Raoul Kopelman
Mar 17, 2012·Colloids and Surfaces. B, Biointerfaces·Ahmet C SabuncuAli Beskok
Apr 12, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Elena GazzanoDario Ghigo
Apr 25, 2012·Annual Review of Biomedical Engineering·Alexandre AlbaneseWarren C W Chan
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 28, 2012·Advanced Drug Delivery Reviews·Veronika MamaevaMika Lindén
Feb 1, 2013·Current Drug Discovery Technologies·Vijay Chandolu, Crispin R Dass
May 2, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ana Lilia Barrán-BerdónAldo Laganà

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Citations

Jan 6, 2016·Scientific Reports·Haibo MuJinyou Duan
Nov 9, 2018·Microscopy Research and Technique·Alexandre LoukanovSeiichiro Nakabayashi

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