Quantification of neuropeptides (calcitonin gene-related peptide, substance P, neurokinin A, neuropeptide Y and vasoactive intestinal polypeptide) expressed in healthy and inflamed human dental pulp

International Endodontic Journal
J Caviedes-BucheliH R Munoz

Abstract

To quantify the expression of calcitonin gene-related peptide (CGRP), substance P (SP), neurokinin A (NKA), neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) in healthy and inflamed human dental pulp tissue. Six pulp samples were obtained from teeth having a clinical diagnosis of acute irreversible pulpitis. Another 12 pulp samples were obtained from premolars where extraction was indicated for orthodontic purposes. In six of these premolar teeth inflammation was induced by mechanical pulp exposure prior to sample collection. All samples were processed and 125I-labelled; neuropeptides were quantified by competition assays. ANOVA and Mann-Whitney's (post hoc) tests were used to establish statistically significant differences between the groups. Expression of five neuropeptides was found in all human pulp samples. Statistical analysis revealed a significantly higher (P < 0.05) expression of CGRP, SP, NKA and NPY in both inflammatory conditions compared with healthy pulp control values. VIP expression remained stable during the inflammatory conditions. Expression of CGRP, SP and NKA released from C-fibres and NPY released from sympathetic fibres is significantly higher in the inflamed human pulp compared with health...Continue Reading

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Citations

Sep 24, 2008·Journal of Dental Research·J L Gibbs, K M Hargreaves
Feb 27, 2016·Clinical Neurology and Neurosurgery·Zhen-long QinJia-xiang Ni
Nov 19, 2013·Journal of Endodontics·Jénifer Martín-GonzálezJuan J Segura-Egea
Jun 22, 2011·Journal of Endodontics·Javier Caviedes-BucheliHugo Roberto Munoz
Apr 2, 2016·International Journal of Dentistry·Gian Marco AbbateLuca Levrini
May 5, 2009·Journal of Endodontics·Javier Caviedes-BucheliHugo Roberto Muñoz
Dec 17, 2008·Journal of Endodontics·Simon A KilloughChris R Irwin
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Nov 21, 2021·International Endodontic Journal·Shehabeldin Mohamed SaberRonald Ordinola-Zapata

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