PMID: 8605218Feb 9, 1996Paper

Quantification of O6-methylguanine-DNA methyltransferase mRNA in human brain tumors

Biochimica Et Biophysica Acta
K MineuraM Kowada

Abstract

O6-Methylguanine-DNA methyltransferase (MGMT) is strongly involved in drug resistance mechanism of tumor cells to chloroethylnitrosoureas (CENUs), because it removes and repairs CENU-induced O6-alkylguanine-DNA by accepting the alkyl group at a cysteine moiety. MGMT activity and MGMT mRNA expression are good indicators for detection of sensitive cells or resistant cells to CENUs. In the present study, we applied a non-radioactive reverse transcription-polymerase chain reaction (RT-PCR) method on quantitative measurement of MGMT mRNA expression. Estimated levels of MGMT mRNA expression determined by this RT-PCR method were consistent with the actual doses of MGMT mRNA. This relationship was noted at a wide range from 10 fg to 10 pg. The relative expression levels of MGMT mRNA estimated from kinetic analysis correlated well with MGMT activity determined using 3H-methyl-nitrosourea-treated DNA substrate in brain tumor cells (P<0.001 with a correlation coefficient of 0.997). The RT-PCR method facilitated quantitative measurements in even a small amount of biopsy specimens obtained by stereotactic brain surgery.

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Citations

Jul 17, 2012·Pituitary·Ann McCormackRoderick Clifton-Bligh
May 5, 2001·Brain Research. Brain Research Reviews·M Bredel
Dec 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J HanssonA Platz
Aug 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jill A MaxwellHenry S Friedman
Aug 22, 2007·International Journal of Cancer. Journal International Du Cancer·Toshio SasajimaKazuo Mizoi
Dec 4, 2003·Expert Opinion on Investigational Drugs·Verena JendrossekMichael Bamberg
Feb 21, 2008·Studies in Family Planning·Saumya RamaRaoJohn W Townsend
Aug 3, 2011·The American Journal of Bioethics : AJOB·Robert M NelsonMary Frances Luce
Oct 17, 2006·Molecular Cancer Therapeutics·Jill A MaxwellHenry S Friedman

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