Quantification of oral mucositis due to radiotherapy by determining viability and maturation of epithelial cells

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
M A StokmanE G E de Vries

Abstract

An in-vitro assay has been developed for quantitative assessment of chemotherapy induced oral mucositis. In the present study this method was evaluated for assessment of irradiation mucositis at a cellular level. Ten patients participated in this consecutive study. All patients were treated with conventional fractionated curative postoperative radiotherapy. Prior to, and weekly during, the irradiation course, oral washings were obtained to determine viability of epithelial cells by trypan blue dye exclusion. Maturation of epithelial cells was assessed from smears (Papanicolaou staining). The viability data were compared with the WHO-score for mucositis. Epithelial cell viability increased during the first three weeks of radiation (P = 0.04), and was seen earlier than the subjective mucosal changes with the WHO-score. Cell maturity shifted from immature and intermediate to mature (P = 0.03). The cell viability assay can be considered an objective method for following the development of irradiation mucositis, and seems to be more sensitive during the first three weeks of irradiation than the WHO-scoring method.

References

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Citations

Sep 15, 2004·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·M A StokmanJ L N Roodenburg
Jan 30, 2007·European Journal of Oncology Nursing : the Official Journal of European Oncology Nursing Society·Karis Kin-Fong Cheng
Feb 23, 2007·Journal of Clinical Nursing·Karis Kin-Fong Cheng
Apr 20, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P M Belfield, A A Dwyer
Nov 11, 2011·International Journal of Oral and Maxillofacial Surgery·A Rodríguez-CaballeroJ L Gutiérrez-Pérez
Sep 4, 2019·Expert Opinion on Drug Metabolism & Toxicology·Ying ZhangQiaojun He
Aug 22, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Srijayaprakash B UppadaSiddappa N Byrareddy
Oct 3, 2018·The Journal of International Medical Research·Xuanxuan WuXuan Zhai

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