Quantification of Pasteurella multocida in experimentally infected pigs using a real-time PCR assay

Research in Veterinary Science
V TocquevilleCorinne Marois-Créhan

Abstract

The aim of the study was to quantify Pasteurella multocida in experimentally infected pigs using a new qPCR assay based on the sodA gene and validated with 35 P. multocida strains, including strains isolated from pigs with pneumonia, clinically healthy pigs (nasal cavities), and human infections. The specificity of the test was verified with a collection of 60 strains of bacterial species other than P. multocida. The estimated detection threshold was 10 genome equivalents per microliter. The amplification efficiency and value of the correlation coefficients were 95.5% (±3.5%) and 0.995 (±0.005), respectively. Analysis of P. multocida suspensions in Buffered Peptone Water Broth and of samples prepared from lungs experimentally spiked with P. multocida revealed detection thresholds of 1.4CFU/μl and 8.4CFU/μl, respectively. In live pigs, experimentally-infected, approximately 10(5), 10(7) and 10(8)genomeequivalents/ml of P. multocida DNA was detected on Day 8 post-infection in the nasal cavities, tonsils and trachea samples, respectively. In dead pigs, approximatively 10(7)genomeequivalents/ml of P. multocida DNA was detected in the lung tissue with pneumonia. The qPCR assay's diagnostic specificity and sensitivity were 100% and 9...Continue Reading

References

May 11, 1990·Nucleic Acids Research·W E BuysM A Smits
Jun 1, 1984·European Journal of Clinical Microbiology·R MuttersW Mannheim
May 23, 1998·Journal of Clinical Microbiology·K M TownsendH J Dawkins
Mar 4, 2000·Veterinary Microbiology·M L HuntK M Townsend
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·B J MayV Kapur
Sep 14, 2001·Letters in Applied Microbiology·J K Miflin, P J Blackall
Jun 18, 2002·Trends in Molecular Medicine·Dieter Klein
Dec 3, 2003·Acta Veterinaria Scandinavica·N F FriisB Kokotovic
Jul 6, 2004·Journal of Microbiological Methods·Dongyou LiuFrank W Austin
May 6, 2005·Journal of Clinical Microbiology·Anne-Lise GautierOlivier Gaillot
Aug 20, 2005·Advances in Physiology Education·Mark A Valasek, Joyce J Repa
Mar 10, 2007·Journal of Microbiological Methods·B G CorneyP J Blackall
Feb 5, 2008·Veterinary Microbiology·Corinne MaroisMarylène Kobisch
Jul 28, 2013·Diagnostic Microbiology and Infectious Disease·Salah ZangenahVolkan Ozenci
Dec 3, 2013·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·C LópezR Terré
Aug 4, 2015·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Eric Dr PondDuncan Webster
Sep 12, 2015·Medicine·Antonio GiordanoDon C Rockey
May 28, 2016·International Journal of Surgery Case Reports·Anders AhlssonJan Källman

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