Quantification of PpIX-fluorescence of cerebral metastases: a pilot study

Clinical & Experimental Metastasis
Johannes KnippsMarcel A Kamp

Abstract

5-ALA fluorescence-guided surgery (FGS) is a major advance in neuro-oncological surgery. So far, Protoporphyrin IX (PpIX)-fluorescence has been observed in about half of cerebral metastases resected with routinely equipped microscopes during 5-ALA FGS. The aim of the present pilot study was to quantify PpIX-induced fluorescence of cerebral metastases with a spectrometer. We hypothesize that non-fluorescing metastases under the operating microscope may have spectrometrically measurable levels of fluorescence. A second aim was to analyze correlations between quantified 5-ALA fluorescence and histology or primary tumor type, respectively. Standard FGS was performed in all patients. The fluorescence intensity of the metastasis was semi-quantitatively determined in vivo by a senior surgeon using a special surgical microscope equipped for FGS. A systematic spectrometric ex vivo evaluation of tumor specimens and PpIX-induced fluorescence was performed using a spectrometer connected by optic fibers to a handheld probe. Quantification of 5-ALA-derived fluorescence was measured in a standardized manner with direct contact between mini-spectrometer and metastasis. The difference between the maximum PpIX-fluorescence at 635 nm and the base...Continue Reading

References

Jan 1, 1980·International Journal of Radiation Oncology, Biology, Physics·B BorgeltF R Hendrickson
Jun 5, 2003·JAMA : the Journal of the American Medical Association·Kaveh G ShojaniaLee Goldman
Oct 31, 2003·Cancer Treatment Reviews·Roy A Patchell
Oct 11, 2005·Journal of Neuro-oncology·Igor T Gavrilovic, Jerome B Posner
Dec 17, 2008·Journal of Neurosurgery·Heon YooSeung Hoon Lee
Sep 10, 2009·Current Opinion in Neurology·Walter Stummer, Marcel A Kamp
Dec 29, 2009·Journal of Neurosurgery·Akash J PatelRaymond Sawaya
Nov 3, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Martin KocherRolf-Peter Mueller
May 12, 2012·Acta neurochirurgica·Marcel A KampMichael Sabel
Aug 10, 2012·Acta neurochirurgica·Marcel A KampMichael Sabel
Jan 12, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Sven O EickerDaniel Hänggi
Oct 3, 2013·Neuro-oncology·Anna S BerghoffMatthias Preusser
Jan 18, 2014·Journal of Neurological Surgery. Part B, Skull Base·Jan Frederick CorneliusHans Jakob Steiger
Jan 29, 2014·Acta neurochirurgica·José Piquer BellochAntonio Cremades
Feb 4, 2014·Neurosurgical Focus·Serge MarbacherJavier Fandino
Apr 10, 2014·Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]·Martin KocherMatthias Guckenberger
Jun 28, 2014·BioMed Research International·José PiquerAntonio Cremades
Jul 15, 2015·Acta neurochirurgica·Marcel A KampMichael Sabel
Aug 19, 2015·Photochemistry and Photobiology·Tetsuya YamamotoAkira Matsumura
Jul 10, 2016·Neurosurgical Review·Marcel A KampMichael Sabel
Nov 14, 2016·World Neurosurgery·Georg EversChristian Ewelt
May 10, 2017·The Lancet Oncology·Michael WellerUNKNOWN European Association for Neuro-Oncology (EANO) Task Force on Gliomas
Oct 27, 2017·World Neurosurgery·Zarela Krause MolleMarcel A Kamp
Mar 21, 2018·Neurosurgical Review·Marcel A KampMichael Sabel

❮ Previous
Next ❯

Citations

Jan 15, 2021·Journal of Clinical Medicine·Alexander J SchupperConstantinos G Hadjipanayis

❮ Previous
Next ❯

Methods Mentioned

BETA
dissection
surgical resection
Fluorescence
surgical resections

Software Mentioned

Igor Spectrometer
Graph Pad Prism
GraphPad

Related Concepts

Related Feeds

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.