PMID: 6968709May 1, 1980Paper

Quantification of proteins with a new sensitive method--zone immunoelectrophoresis assay

Hoppe-Seyler's Zeitschrift für physiologische Chemie
O Vesterberg

Abstract

Quantitative determination of proteins has been made by electrophoresis thereof in tubular canals filled with agarose gel containing antibodies. The procedure is simple and practicable. Sample volumes of a few microliter up to some hundred microliter may be added for each test. The amount of protein in a sample was found to be linearly related to the distance from the point of application (i.e. gel surface) to the front of the immunoprecipitation zone. The zone immunoelectrophoresis method shows over rocket immunoelectrophoresis the following advantages: less consumption of antibodies; much lower detection limit (0.05 microgram of protein per ml); larger concentration range with a linear calibration curve. A high correlation (0.98) between the first- and last-mentioned method was obtained for alpha 1-antitrypsin. Precision data are given for albumin and alpha-fetoprotein and typically a relative standard deviation of a few percent was obtained.

Citations

Sep 1, 1988·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·W MärzW Gross
Dec 1, 1993·Electrophoresis·O Vesterberg
Jan 1, 1993·International Archives of Occupational and Environmental Health·L HolmquistB Persson
Oct 20, 1989·Journal of Chromatography·O Vesterberg
Oct 15, 1996·Journal of Biochemical and Biophysical Methods·L HolmquistO Vesterberg
Apr 30, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·L Holmquist, O Vesterberg
Nov 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·W März, W Gross
Dec 1, 1991·Journal of Biochemical and Biophysical Methods·L Holmquist, O Vesterberg

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