Quantification of Pseudomonas aeruginosa in multispecies biofilms using PMA-qPCR

PeerJ
Sarah Tavernier, T Coenye

Abstract

Multispecies biofilms are an important healthcare problem and may lead to persistent infections. These infections are difficult to treat, as cells in a biofilm are highly resistant to antimicrobial agents. While increasingly being recognized as important, the properties of multispecies biofilms remain poorly studied. In order to do so, the quantification of the individual species is needed. The current cultivation-based approaches can lead to an underestimation of the actual cell number and are time-consuming. In the present study we set up a culture-independent approach based on propidium monoazide qPCR (PMA-qPCR) to quantify Pseudomonas aeruginosa in a multispecies biofilm. As a proof of concept, we explored the influence of the combined presence of Staphylococcus aureus, Streptococcus anginosus and Burkholderia cenocepacia on the antimicrobial susceptibility of P. aeruginosa using this PMA-qPCR approach.

References

Aug 1, 1965·Journal of Molecular Biology·M J Waring
Dec 18, 2004·Cancer Research·Akihisa TakahashiTakeo Ohnishi
Jun 5, 2007·Journal of Microbiological Methods·Andreas NockerAnne K Camper
Jan 16, 2008·Antimicrobial Agents and Chemotherapy·Jaeeun KimJeyong Yoon
Nov 4, 2008·The Journal of Hospital Infection·E PeetersT Coenye
Dec 24, 2008·Journal of Microbiological Methods·Andreas NockerRoland Brousseau
Jan 23, 2010·Journal of Medical Microbiology·Christopher D SibleyMichael G Surette
Jun 29, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Heike Sträuber, Susann Müller
Jul 20, 2010·Journal of Applied Microbiology·L M E VanheeT Coenye
Jul 10, 2012·International Journal of Antimicrobial Agents·Susana Patrícia LopesMaria Olívia Pereira
Sep 4, 2012·Journal of Microbiological Methods·Mariana FittipaldiFrancesc Codony
Feb 20, 2014·Journal of Neurovirology·John R KeltnerUNKNOWN CHARTER Group
Jun 12, 2014·Frontiers in Microbiology·Laam LiSebastien P Faucher

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Citations

Mar 1, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·R Shwetharani, R Geetha Balakrishna
Nov 22, 2016·Critical Reviews in Microbiology·Joana AzeredoClaus Sternberg
Jul 12, 2017·Antimicrobial Agents and Chemotherapy·Sarah TavernierTom Coenye
Sep 7, 2017·Applied Microbiology and Biotechnology·B ReynekeW Khan
Sep 22, 2019·Scientific Reports·Andreia Patrícia MagalhãesNuno Cerca
Dec 3, 2016·Chembiochem : a European Journal of Chemical Biology·Yu LuoFionnuala T Lundy
Jul 10, 2019·European Respiratory Review : an Official Journal of the European Respiratory Society·Eva VandeplasscheAurélie Crabbé
Jun 24, 2018·Scientific Reports·Susana Patrícia LopesMaria Olívia Pereira

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Methods Mentioned

BETA
PMA
electrophoresis
PCR

Software Mentioned

SPSS

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