Abstract
The aim of this study was to establish and validate an alternative high-performance liquid chromatography method for simultaneous quantification of pyrazinamide, isoniazid, acetyl-isoniazid and rifampicin in plasma of patients under treatment for tuberculosis. The performed method was lineal (r2 > 0.99) in the range of 2.00-50.00 μg/mL for pyrazinamide, 0.50-20.00 μg/mL for both acetyl-isoniazid and isoniazid, and 1.20-25.00 μg/mL for rifampicin. Precision and trueness were demonstrated with coefficient of variation < 15% and deviations < 15%, respectively, for quality controls samples. The lower limits of quantification were 2.00, 0.50, 0.50, and 1.20 μg/mL for pyrazinamide, isoniazid, acetyl-isoniazid and rifampicin, respectively. The method was applied for the analysis of plasma from patients with tuberculosis. This method allowed ensuring reliable quantification of the target compounds and their pharmacokinetics parameters. In general, the mean values of maximum concentration of each antituberculosis drug were located within their respective reference therapeutic ranges. However, patients with sub-therapeutic plasma concentrations of isoniazid and rifampicin were detected. This is the first analytical technique that simult...Continue Reading
References
Dec 15, 2004·Chest·Charles Peloquin
May 17, 2007·European Journal of Clinical Pharmacology·P R DonaldJ S Maritz
Jun 9, 2007·Journal of Chromatographic Science·Ming YanYin Sui
Feb 29, 2008·Journal of Separation Science·Dao T-T NguyenJean-Luc Veuthey
Feb 12, 2009·International Ophthalmology·Katherine Anne Talbert Estlin, Alfredo A Sadun
Dec 30, 2009·Antimicrobial Agents and Chemotherapy·Rovina RuslamiReinout van Crevel
Jul 28, 2010·Antimicrobial Agents and Chemotherapy·Marc WeinerAndrew Vernon
Nov 17, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zhifeng ZhouFei Zou
Jun 18, 2011·Bioanalysis·Steve LowesDennis Heller
Jan 2, 2013·Molecular Biology Reports·Haijian DuWenjie Huang
May 1, 2013·Antimicrobial Agents and Chemotherapy·Alma TostmannRob E Aarnoutse
Nov 10, 2013·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·F FahimiJ Salamzadeh
Oct 21, 2014·Journal of Separation Science·Wei LiLiping Yang
May 27, 2015·The Journal of Infectious Diseases·Tawanda GumboSantiago Ferrer-Bazaga
Jun 10, 2015·Antimicrobial Agents and Chemotherapy·Marieke G G SturkenboomJan-Willem C Alffenaar
Oct 27, 2015·PloS One·Paolo DentiAase Bengaard Andersen
Mar 8, 2016·Expert Opinion on Drug Metabolism & Toxicology·Marlanka A ZuurJan-Willem C Alffenaar
May 20, 2016·Journal of Separation Science·Heli SirénTamara Tuuminen
Dec 13, 2016·Journal of Pharmaceutical and Biomedical Analysis·Kanumuri Siva Rama RajuMuhammad Wahajuddin
Mar 1, 2017·Antimicrobial Agents and Chemotherapy·Geetha RamachandranSoumya Swaminathan