Quantification of rhBMP2 in bioactive bone materials

Regenerative Biomaterials
Huan LianChunren Wang

Abstract

Bone morphogenetic protein (BMP), belongs to transforming growth factor-β (TGF-β) superfamily except BMP-1. Implanting BMP into muscular tissues induces ectopic bone formation at the site of implantation, which provides opportunity for the treatment of bone defects. Recombinant human BMP-2 (rhBMP-2) has been used clinically, but the lack of standard methods for quantifying rhBMP-2 biological activity greatly hindered the progress of commercialization. In this article, we describe an in vitro rhBMP-2 quantification method, as well as the data analyzation pipeline through logistic regression in RStudio. Previous studies indicated that alkaline phosphatase (ALP) activity of C2C12 cells was significantly increased when exposed to rhBMP-2, and showed dose-dependent effects in a certain concentration range of rhBMP-2. Thus, we chose to quantify ALP activity as an indicator of rhBMP-2 bioactivity in vitro. A sigmoid relationship between the ALP activity and concentration of rhBMP-2 was discovered. However, there are tons of regression models for such a non-linear relationship. It has always been a major concern for researchers to choose a proper model that not only fit data accurately, but also have parameters representing practical m...Continue Reading

References

Aug 1, 1987·Clinical Biochemistry·D W Moss
May 18, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M F Sciadini, K D Johnson
Aug 20, 2003·Journal of Biomedical Materials Research. Part a·Toshihiro MatsuoTeruo Murakami
Dec 29, 2004·Growth Factors·Di ChenGregory R Mundy
Sep 30, 2008·Journal of Immunological Methods·Rod A HermanGuomin Shan
Jun 3, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Deepak Bushan RainaAshok Kumar

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Methods Mentioned

BETA
ELISA

Software Mentioned

RStuio
MASS
R script
drc
RStudio

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