Quantification of Shiga toxin-converting bacteriophages in wastewater and in fecal samples by real-time quantitative PCR.

Applied and Environmental Microbiology
Lejla ImamovicMaite Muniesa

Abstract

Shiga toxin-converting bacteriophages (Stx phages) are involved in the pathogenicity of some enteric bacteria, such as Escherichia coli O157:H7. Stx phages are released from their bacterial hosts after lytic induction and remain free in the environment. Samples were analyzed for the presence of free Stx phages by an experimental approach based on the use of real-time quantitative PCR (qPCR), which enables stx to be detected in the DNA from the viral fraction of each sample. A total of 150 samples, including urban raw sewage samples, wastewater samples with fecal contamination from cattle, pigs, and poultry, and fecal samples from humans and diverse animals, were used in this study. Stx phages were detected in 70.0% of urban sewage samples (10 to 10(3) gene copies [GC] per ml) and in 94.0% of animal wastewater samples of several origins (10 to 10(10) GC per ml). Eighty-nine percent of cattle fecal samples were positive for Stx phages (10 to 10(5) GC per g of sample), as were 31.8% of other fecal samples of various origins (10 to 10(4) GC per g of sample). The stx(2) genes and stx(2) variants were detected in the viral fraction of some of the samples after sequencing of stx(2) fragments amplified by conventional PCR. The occurren...Continue Reading

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Citations

Mar 20, 2013·Environmental Science and Pollution Research International·Ana Rita Varela, Célia M Manaia
Aug 3, 2011·Antimicrobial Agents and Chemotherapy·Marta Colomer-LluchMaite Muniesa
Apr 17, 2012·Applied and Environmental Microbiology·Maite MuniesaHarald Brüssow
Jun 12, 2013·Applied and Environmental Microbiology·Alexandre Martinez-CastilloMaite Muniesa
Mar 29, 2011·Applied and Environmental Microbiology·Lejla Imamovic, Maite Muniesa
Jun 16, 2012·PloS One·Neelanjana GhoshJordi Bella
Aug 27, 2014·Future Microbiology·Maite Muniesa, Juan Jofre
Feb 18, 2016·World Journal of Microbiology & Biotechnology·Alba BlánquezManuel Hernández
May 4, 2011·Microbial Biotechnology·Maite MuniesaJuan Jofre
Feb 1, 2012·Environmental Microbiology Reports·Alexandre Martínez-CastilloMaite Muniesa
Dec 3, 2015·Environmental Microbiology·Lejla ImamovicMaite Muniesa
Aug 26, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Hege S TunsjøTruls M Leegaard
Nov 10, 2011·Toxins·Steven A Mauro, Gerald B Koudelka
Nov 23, 2017·Frontiers in Microbiology·Anna KłopotKrystyna Dąbrowska
Aug 6, 2017·Applied and Environmental Microbiology·Yuan FangMichael G Gänzle
Jan 28, 2014·Applied and Environmental Microbiology·Anna Allué-GuardiaMaite Muniesa
Aug 17, 2019·Environmental Microbiology·Xiaoxiao LiuXiaoxue Wang
Apr 1, 2017·Journal of Water and Health·Nicole M MastersMohammad Katouli
Jun 18, 2021·Microbial Ecology·Aparna NairKrishna Khairnar
Mar 22, 2014·ACS Applied Materials & Interfaces·Jin-Hee HanIan M Kennedy

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