Quantification of sugars and organic acids in tomato fruits

MethodsX
Carlos AgiusWilfried Rozhon

Abstract

Sugar and organic acid contents are major factors for tomato fruit flavour and are important breeding traits. Here we provide an improved protocol for accurate quantification of the main sugars, glucose and fructose, and the organic acids, citric acid and malic acid, present in tomato. The tomato extract is spiked with lactose and tricarballylic acid as internal standards and loaded onto a NH2 solid phase extraction (SPE) column. The sugars appear in the flow-through and are subsequently analysed by HPLC using a Nucleodur NH2 column and a refractive index detector. The organic acids bind to the SPE column and are eluted with 400 mM phosphoric acid. For analysis, the organic acids are separated by HPLC using a Nucleodur C18ec column and detected by UV absorption at 210 nm. The method shows excellent inter-day and intra-day reproducibility for glucose, fructose and citric acid with standard deviations of 1-5%. Quantification of citric acid by HPLC and GC-MS showed perfect agreement with a deviation of less than 3%. •Simple method for quantification of glucose, fructose, citric acid and malic acid in tomato.•Efficient removal of interfering compounds by solid phase extraction.•High intra and inter-day reproducibility.

Citations

Jun 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yingying HanMiaomiao Li
Apr 6, 2020·Journal of the Science of Food and Agriculture·Leontina LipanÁngel A Carbonell-Barrachina
Oct 18, 2019·Critical Reviews in Food Science and Nutrition·Supradip SahaKaushik Banerjee
Jun 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carlos AgiusWilfried Rozhon
Dec 24, 2018·Foods·Carolina Cortés-HerreraFabio Granados-Chinchilla
Apr 23, 2019·Journal of the Science of Food and Agriculture·Ginés IbáñezSalvador Roselló
Jan 29, 2020·International Journal of Biological Macromolecules·Pinyi GaoXuegui Liu
Sep 18, 2021·Journal, Genetic Engineering & Biotechnology·Adekunle Olusegun Adeoye, Agbaje Lateef
Dec 21, 2021·Analytical and Bioanalytical Chemistry·Martin MeyerOliver J Schmitz
Dec 30, 2021·Magnetic Resonance in Chemistry : MRC·Rodolphe LeforestierMaja Musse

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Methods Mentioned

BETA
NMR
solid phase extraction
reversed
ion exclusion chromatography

Software Mentioned

Clarity

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