Quantification of Sunscreen Benzophenone-4 in Hair Shampoos by Hydrophilic Interactions Thin-Layer Chromatography/Densitometry or Derivative UV Spectrophotometry

Journal of Analytical Methods in Chemistry
Anna W SobanskaElżbieta Brzezińska

Abstract

Benzophenone-4 (BZ4) was separated from surfactants, dyes, preservatives, and other components of hair shampoos by thin-layer chromatography on silica gel 60 stationary phase, with ethyl acetate-ethanol-water-pH 6 phosphate buffer (15 : 7 : 5 : 1 v/v/v/v) as mobile phase. Densitometry scanning of chromatograms was performed at 285 nm. The densitometric calibration curve for BZ4 was nonlinear (second-degree polynomial), with R > 0.999. The limits of detection and quantification were ca. 0.03 and ca. 0.1 μg spot(-1), respectively. The results obtained by HPTLC-densitometry were compared to those obtained by zero and 2nd derivative UV spectrophotometry. In the case of spectrophotometric methods, calibration curves were linear with R > 0.9998. The chromatographic method was fully validated.

References

Mar 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·P G PiettaM Carini
Oct 28, 1999·International Journal of Pharmaceutics·G PotardJ P Marty
Nov 30, 2000·Skin Pharmacology and Applied Skin Physiology·F Benech-KiefferH Schaefer
Nov 29, 2002·Journal of Chromatography. a·Alberto Chisvert, Amparo Salvador

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Citations

Jul 5, 2016·Food Chemistry·Honorio Vergara-DomínguezMaría Roca
Jan 6, 2021·Contact Dermatitis·Chayada ChaiyabutrWaranya Boonchai
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Izabela Narloch, Grażyna Wejnerowska

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