Quantification of surface etching by common buffers and implications on the accuracy of label-free biological assays

Biosensors & Bioelectronics
Sunmin AhnM Selim Unlü

Abstract

High throughput analyses in biochemical assays are gaining popularity in the post-genomic era. Multiple label-free detection methods are especially of interest, as they allow quantitative monitoring of biomolecular interactions. It is assumed that the sensor surface is stable to the surrounding medium while the biochemical processes are taking place. Using the Interferometric Reflectance Imaging Sensor (IRIS), we found that buffers commonly used in biochemical reactions can remove silicon dioxide, a material frequently used as the solid support in the microarray industry. Here, we report 53 pm to 731 pm etching of the surface silicon oxide over a 12-h period for several different buffers, including various concentrations of SSC, SSPE, PBS, TRIS, MES, sodium phosphate, and potassium phosphate buffers, and found that PBS and MES buffers are much more benign than the others. We observe a linear dependence of the etch depth over time, and we find the etch rate of silicon dioxide in different buffers that ranges from 2.73±0.76 pm/h in 1M NaCl to 43.54±2.95 pm/h in 6×SSC. The protective effects by chemical modifications of the surface are explored. We demonstrate unaccounted glass etching leading to erroneous results with label-free ...Continue Reading

References

Dec 6, 2001·Nature Reviews. Genetics·A C Syvänen
Nov 28, 2002·Nature Genetics·Gavin MacBeath
Aug 11, 2004·Analytical Biochemistry·Marina CretichMarcella Chiari
Jun 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Emre OzkumurM Selim Unlü
Jul 1, 2008·Nature Methods·Frank Vollmer, Stephen Arnold
Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patricia M DoveJames J De Yoreo
Feb 18, 2009·Analytical and Bioanalytical Chemistry·Abraham J QaviRyan C Bailey
Jul 25, 2009·Biosensors & Bioelectronics·Emre OzkumurM Selim Unlü
Dec 17, 2009·Analytical Chemistry·Stella H NorthChris R Taitt
Jul 20, 2010·Annual Review of Analytical Chemistry·Kyo Seon HwangTae Song Kim

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Citations

Nov 26, 2015·Talanta·Chiara FinettiMarcella Chiari
Oct 16, 2012·Biophysical Journal·Sunmin AhnRostem J Irani
Mar 16, 2016·IEEE Transactions on Nanobioscience·Zongjie WangKeekyoung Kim
Jun 22, 2016·Journal of Neural Engineering·Xin LeiDaniel Palanker
Dec 24, 2018·Micromachines·Pranav AmbhorkarDong-Il Dan Cho
Oct 1, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Yuanchao LiJan Genzer
Apr 4, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Sunmin AhnM Selim Ünlü

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