Quantification of TGF-ß1, PDGF and IGF-1 cytokine expression after fracture treatment vs. non-union therapy via masquelet

Injury
Christian FischerArash Moghaddam

Abstract

Biochemical processes during bone regeneration can be analysed via quantification of peripheral serum cytokine levels. To date, serum levels of cytokines in patients treated with masquelet technique and patients with normal bone healing have not been compared. This comparison is supposed to deliver novel insights into the process of bone regeneration. Our aim was to validate this established method in the monitoring of bone regeneration after non-union treatment in masquelet technique. Between 04/2008 and 01/2014 three groups were recruited: G1 (10 patients) with long bone non-unions, treated successfully with masquelet therapy, G2 (6 patients) with unsuccessful masquelet therapy and G3 (10 patients) with long bone fractures and normal bone healing. Peripheral blood samples were collected over a period of six months following a standardised time pattern in combination with clinical and radiologic follow up. TGF-ß1, PDGF-AB and IGF-1 were measured using commercially available immunoassays. TGF-ß1 levels in G1 and G2 demonstrated a parallel and lower overall concentration over time compared to G3. G3 showed a significant TGF-ß1 peak 2 weeks after surgery compared to G1 (p=0.0054). PDGF-AB concentrations were always lower in G2 th...Continue Reading

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Citations

Oct 5, 2016·Injury·Cyril MauffreyAlain-Charles Masquelet
Mar 3, 2017·World Journal of Orthopedics·Enrique Guerado, Enrique Caso
Nov 20, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jennifer A McKenzieMegan L Killian
Jan 1, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Andrea I AlfordSarah McBride-Gagyi
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui I FalachoFernando Guerra
Aug 17, 2021·Journal of Orthopaedic Surgery·Kabilan ThurairajahZsolt J Balogh

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