PMID: 8605069Oct 1, 1995Paper

Quantification of the ease of removal of bacteria from surfaces

Journal of Industrial Microbiology
P J EgintonP Gilbert

Abstract

This paper describes a technique which reproducibly quantifies the ease of removal of microorganisms from surfaces. Tiles (22 mm x 22 mm) of various materials were colonised with Staphylococcus epidermidis NCTC 11047, Escherichia coli K12 HB101 or Pseudomonas aeruginosa PaWH, by submersion, for various times (2 min-48 h), in inoculated Tryptone Soya broth (37 degrees C). Colonised tiles were blotted onto a Tryptone Soya agar plate for 1 min and the process was repeated through a succession of agar plates. The final plate contained tetrazolium salts (0.05% w/v) and was incubated in situ with the tile. Tetrazolium plates indicated that very few organisms remained on the tiles after 15 successive blots. In all instances, the number of recovered colonies per plate decreased exponentially with plate succession number, according to the relationship, CFU = A.10-kN, where CFU is the number of colonies transferred, k is the removal exponent, A is the intercept and N is the plate succession number. Removal exponents differed significantly between organisms (P > 0.95), depended on the nature of the test surface, and decreased as the inital attachment and colonisation time was increased from 2 min-48 h. Intercept values (A) but not the gra...Continue Reading

References

Jan 1, 1975·Annual Review of Microbiology·R J Gibbons, J V Houte
Jul 1, 1992·Antimicrobial Agents and Chemotherapy·H AnwarJ W Costerton
May 1, 1991·Antimicrobial Agents and Chemotherapy·B GiwercmanJ W Costerton
Jul 1, 1990·The Journal of Antimicrobial Chemotherapy·B D HoyleJ W Costerton
Nov 1, 1989·The Journal of Antimicrobial Chemotherapy·H AnwarJ W Costerton
Dec 1, 1988·The Journal of Antimicrobial Chemotherapy·M R BrownP Gilbert
Jan 1, 1987·Annual Review of Microbiology·J W CostertonT J Marrie
Jan 1, 1981·Annual Review of Microbiology·J W CostertonK J Cheng
Sep 1, 1981·Canadian Journal of Microbiology·W F McCoyJ W Costerton
May 1, 1994·Trends in Microbiology·T B May, A M Chakrabarty
Jul 1, 1943·Journal of Bacteriology·C E Zobell

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Citations

Jun 9, 2004·Bioresource Technology·Andrea LomanderLaila Ali
Jul 10, 2001·Journal of Applied Microbiology·J BarkerS F Bloomfield
Jul 31, 2002·Applied and Environmental Microbiology·Graziella Midelet, Brigitte Carpentier
Oct 31, 2012·International Journal of Food Microbiology·Olivier FirmesseBrigitte Carpentier
May 19, 2010·Letters in Applied Microbiology·R N HaddadinA A Shehabi
Oct 15, 2014·Asian Pacific Journal of Tropical Medicine·Victoria O AdetunjiJacob Kwaga
Oct 22, 2016·International Journal of Food Microbiology·Anaïs OverneyOlivier Firmesse
Aug 9, 2011·Journal of Food Protection·Rebecca M Goulter-ThorsenGary A Dykes
Feb 8, 2005·Journal of Food Protection·Anne-Marie Revol-JunellesJean-Bernard Millière
Sep 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·P Sautrot-BaD-L Versace
Jul 27, 2017·Frontiers in Microbiology·Eleni N GkanaGeorge-John E Nychas
Mar 28, 2002·Ecotoxicology and Environmental Safety·K Park, S Q Hee

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