Quantification of TSPO overexpression in a rat model of local neuroinflammation induced by intracerebral injection of LPS by the use of [(18)F]DPA-714 PET

European Journal of Nuclear Medicine and Molecular Imaging
Dieter OryCindy Casteels

Abstract

[(18)F]DPA-714 is a radiotracer with high affinity for TSPO. We have characterized the kinetics of [(18)F]DPA-714 in rat brain and evaluated its ability to quantify TSPO expression with PET using a neuroinflammation model induced by unilateral intracerebral injection of lipopolysaccharide (LPS). Dynamic small-animal PET scans with [(18)F]DPA-714 were performed in Wistar rats on a FOCUS-220 system for up to 3 h. Both plasma and perfused brain homogenates were analysed using HPLC to quantify radiometabolites. Full kinetic modelling of [(18)F]DPA-714 brain uptake was performed using a metabolite-corrected arterial plasma input function. Binding potential (BPND) calculated as the distribution volume ratio minus one (DVR-1) between affected and healthy brain tissue was used as the outcome measure and evaluated against reference tissue models. The percentage of intact [(18)F]DPA-714 in arterial plasma samples was 92 ± 4 % at 10 min, 75 ± 8 % at 40 min and 52 ± 6 % at 180 min. The radiometabolite fraction in brain was negligible (<3 % at 30 min). Among the models investigated, the reversible two-tissue (2T) compartment model best described [(18)F]DPA-714 brain kinetics. BPND values obtained with a simplified and a multilinear referenc...Continue Reading

References

Jan 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R E CarsonP Herscovitch
Jan 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A A LammertsmaR S Frackowiak
Dec 1, 1996·NeuroImage·A A Lammertsma, S P Hume
Sep 16, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Masanori IchiseRichard E Carson
May 24, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Robert B InnisRichard E Carson
Apr 1, 2008·Pharmacology & Therapeutics·Ming-Kai Chen, Tomás R Guilarte
Apr 17, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Michelle L JamesMichael Kassiou
Sep 23, 2008·Archives of Pharmacal Research·Kyung Hee Yang, Myung Gull Lee
Dec 5, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Sriram VennetiJulia Kofler
Feb 19, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Fabien ChauveauBertrand Tavitian
Feb 19, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Rebecca L McNameeChester A Mathis
Mar 31, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Janine DoorduinErik F J de Vries
Apr 3, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Maqsood YaqubAdriaan A Lammertsma
Nov 21, 2009·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Y K ChoM G Lee
Mar 23, 2010·Cell·Christopher K GlassFred H Gage
Dec 17, 2011·Nuclear Medicine and Biology·Nicolas ArlicotDenis Guilloteau
Jan 19, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Dewei TangH Charles Manning
May 17, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Maqsood YaqubRonald Boellaard
Jul 31, 2012·Neurobiology of Aging·Alie SchuitemakerBart N M van Berckel
Oct 16, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Marie-Anne PeyronneauHéric Valette
Nov 10, 2012·EJNMMI Research·Stefanie DedeurwaerdereMarie-Claude Grégoire
Jan 10, 2013·PloS One·Philippe CorciaMaria-Joao Ribeiro
Feb 12, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Alex M DickensLaura Airas
Apr 2, 2014·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Keun-Ryung BaeSeong-Woon Yu
Jun 19, 2014·Current Pharmaceutical Design·Dieter OryGuy Bormans
Jul 10, 2014·EJNMMI Research·Maria-Joao RibeiroDenis Guilloteau
Nov 27, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Cristian A SalinasRoger N Gunn
Dec 3, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Ling FengLars H Pinborg
Dec 10, 2014·European Journal of Nuclear Medicine and Molecular Imaging·S LavisseP Hantraye
Feb 5, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sandeep S V GollaJuha O Rinne

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Citations

Jan 25, 2016·Psychopharmacology·Anindya BhattacharyaWayne C Drevets
Aug 3, 2016·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Sujata SridharanHervé Boutin
Feb 11, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Tina Notter, Urs Meyer
Jul 12, 2017·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Lien BeckersMyriam Baes
Jan 12, 2019·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Tamara BerdyyevaAnna Katrin Szardenings
Mar 2, 2021·Acta Pharmaceutica Sinica. B·Lingling ZhangSteven H Liang
Sep 29, 2019·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Donatienne Van WeehaegheKoen Van Laere
May 31, 2021·Brain, Behavior, and Immunity·Marta Vicente-RodríguezChristine A Parker
Jul 11, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Nadja Van CampHervé Boutin

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