Quantification of tyrosinase, TRP-1, and Trp-2 transcripts in human melanocytes by reverse transcriptase-competitive multiplex PCR--regulation by steroid hormones

The Journal of Investigative Dermatology
S KippenbergerR Kaufmann

Abstract

We have introduced a reverse transcriptase polymerase chain reaction based method to measure mRNA levels of the melanogenesis enzymes tyrosinase, tyrosinase-related-protein 1 (TRP-1), and tyrosinase-related-protein 2 (TRP-2). Expression was determined by reverse transcriptase-competitive multiplex polymerase chain reaction of (i) melanogenesis enzyme transcripts and the "housekeeping" gene glyceraldehyde-3-phosphate dehydrogenase, and (ii) two internal standards consisting of mutated melanogenesis enzyme cDNA and mutated gene glyceraldehyde-3-phosphate dehydrogenase cDNA. This was investigated on in vitro cultured melanocytes in the presence of three different steroids; one glucocorticoid (betamethasone-17-valerate) and two sex steroids (diethylstilbestrol and estradiol). All three steroids lead to an increase of about 1.5-2.5-fold of tyrosinase transcripts. The amount of TRP-1 transcripts was likewise enhanced, but only moderately (approximately 1.5-fold). In contrast, TRP-2 transcripts were reduced by approximately 40% in number after betamethasone-17-valerate treatment, whereas the two sex steroids, diethylstilbestrol and estradiol, caused an upregulation of about 20-fold of the initial TRP-2 transcript level. We therefore s...Continue Reading

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Citations

Jun 17, 2009·Archives of Dermatology·Mary Alice NadingDarrel L Ellis
Jun 8, 2012·Journal of Cosmetic Dermatology·Vasanop VachiramonKunlawat Thadanipon
Mar 27, 2010·Journal of the European Academy of Dermatology and Venereology : JEADV·Y H JangY C Kim
Apr 14, 2010·Journal of the European Academy of Dermatology and Venereology : JEADV·W ChenJ Ring
Mar 13, 2014·Pigment Cell & Melanoma Research·Reinhart SpeeckaertNanja van Geel
Aug 1, 2015·Pigment Cell & Melanoma Research·Ai-Young Lee
Jan 26, 2010·Actas dermo-sifiliográficas·H Young Kang, J-P Ortonne
May 28, 2009·Experimental Dermatology·Karin U SchallreuterJohn M Wood
Sep 28, 2010·American Journal of Obstetrics and Gynecology·Dayna G Diven, Jessica M Crawford
Dec 3, 2014·Ageing Research Reviews·Yan PengBiao Cheng
Jul 21, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M S M V SartiA M L Castrucci
Oct 16, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Arno HueberPeter J Esser
Sep 24, 2004·Physiological Reviews·Andrzej SlominskiJacobo Wortsman
Jan 24, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gertrude-E Costin, Vincent J Hearing
Apr 12, 2013·International Journal of Cosmetic Science·T MatamáA C Gomes

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