Quantification of unintegrated HIV-1 DNA at the single cell level in vivo.

PloS One
Rodolphe Suspène, Andreas Meyerhans

Abstract

In the nucleus of HIV-1 infected cells, unintegrated HIV-1 DNA molecules exist in the form of one and two LTR circles and linear molecules with degraded extremities. In tissue culture they are invariably more numerous than the provirus, the relative proportion of integrated to unintegrated forms varies widely from ∼1:1 to 1:10 and even over 1:100. In vivo, this ratio is unknown. To determine it, single nuclei from two infected patients with a known provirus copy number were microdissected, HIV DNA was amplified by nested PCR, cloned and individual clones sequenced. Given the extraordinary sequence complexity, we made the assumption that the total number of distinct sequences approximated to real number of amplifiable HIV-1 DNA templates in the nucleus. We found that the number of unintegrated DNA molecules increased linearly with the proviral copy number there being on average 86 unintegrated molecules per provirus.

References

Dec 1, 1991·Journal of Virology·C M Farnet, W A Haseltine
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M InoueE P Reddy
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P O BrownJ M Bishop
May 8, 1987·Cell·P O BrownJ M Bishop
Mar 9, 1995·Nature·D L RobertsonB H Hahn
Nov 26, 1997·Journal of Virology·D P WooleyR C Desrosiers
Dec 29, 1999·Nature Medicine·S C Ray, T C Quinn
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GrattonS Wain-Hobson
Aug 16, 2001·AIDS·M HoelscherUNKNOWN UNAIDS Network for HIV Isolation and Characterization
Jul 12, 2002·Nature·Andreas JungAndreas Meyerhans
May 4, 2004·Nucleic Acids Research·Rodolphe SuspèneJean-Pierre Vartanian
Aug 7, 2004·Current Biology : CB·Kate N BishopMichael H Malim
Nov 22, 2005·The Journal of General Virology·Marie-Jeanne DumaurierRémi Cheynier
Nov 28, 2006·Virology·Hongzhan XuVinay K Pathak
Jun 5, 2010·PLoS Pathogens·Jean-Pierre VartanianSimon Wain-Hobson

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Citations

Jun 13, 2015·Cell Host & Microbe·Andrew P Rice, Jason T Kimata
May 7, 2016·Viruses·Angela CiuffiAmalio Telenti
Apr 13, 2017·Current HIV/AIDS Reports·Radwa R Sharaf, Jonathan Z Li
Mar 5, 2017·Virus Genes·Bizhan Romani, Elham Allahbakhshi
Sep 21, 2013·Journal of Virology·Benjamin TrinitéDavid N Levy
Sep 21, 2013·Journal of Virology·E Carol McWilliam Leitch, John McLauchlan
Sep 29, 2015·PloS One·Sieberth Nascimento-BritoLuiz Mário R Janini
May 18, 2017·Journal of Neurovirology·Weston C RodaChristopher Power

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Methods Mentioned

BETA
Fluorescence
PCR

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