Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Nancy CooperJean W Lee

Abstract

A simple HPLC method was developed and validated for the determination of uric acid (UA), xanthine (X) and hypoxanthine (HX) concentrations in human serum to support pharmacodynamic (PD) studies of a novel xanthine oxidase inhibitor during its clinical development. Serum proteins were removed by ultrafiltration. The hydrophilic analytes and the I.S. were eluted by 100% aqueous phosphate buffer mobile phase. The hydrophobic matrix components (late peaks) were eluted with a step gradient of a higher organic mobile phase. Validation on linearity, sensitivity, precision, accuracy, stability, and robustness of the method for PD biomarkers (UA, X, and HX) was carried out in a similar manner to that for pharmacokinetic (PK) data where applicable. Issues of selectivity for endogenous biomarker analytes and individual concentration variations were addressed during method validation. Standards were prepared in analyte-free phosphate buffer. Quality control samples were prepared in control serum from individuals not dosed with the xanthine oxidase inhibitor. The method was simple and robust with good accuracy and precision for the measurement of serum UA, X, and HX concentrations.

References

Jan 1, 1979·The International Journal of Biochemistry·A McHaleM P Coughlan
Nov 1, 1991·Journal of Leukocyte Biology·M SuzukiD N Granger
Jan 1, 1989·Free Radical Biology & Medicine·J S BeckmanB A Freeman
May 1, 1983·Clinical Pharmacology and Therapeutics·D M GrantW Kalow
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W F PetroneJ M McCord
Aug 11, 1995·The Journal of Biological Chemistry·Y Xia, J L Zweier
May 1, 1995·Journal of Clinical Pharmacology·J W LeeW A Colburn
Aug 7, 1993·Biochimica Et Biophysica Acta·Y MoriwakiK Higashino
Jun 7, 1996·Journal of Chromatography. B, Biomedical Applications·T YamamotoK Higashino
Mar 28, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M Czauderna, J Kowalczyk
Apr 27, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·M G BattelliF Stirpe
Jul 7, 1999·Journal of Biochemical and Biophysical Methods·A K BhargavaC S Pundir
Mar 9, 2000·Journal of Pharmaceutical and Biomedical Analysis·J W FindlayR R Bowsher
Sep 14, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·M Czauderna, J Kowalczyk
Nov 22, 2000·Brain Research. Brain Research Protocols·A AtlanteS Passarella
Apr 17, 2001·Pharmaceutical Research·V P ShahA Yacobi
Jun 14, 2001·Journal of Clinical Pharmacology·S al-AlaiyanA el-Yazigi
Jul 13, 2001·The American Journal of Cardiology·P PatetsiosT F Panetta
Oct 31, 2001·Pharmaceutical Research·K J MillerR S Weiner
Nov 7, 1996·Chemical Reviews·Russ Hille
Jan 1, 1965·Clinica Chimica Acta; International Journal of Clinical Chemistry·U A AL-KHALIDIM H SHAMMAA
Jan 1, 1996·Analytical Chemistry·Z LiS Dong

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Citations

Mar 19, 2014·Enzyme and Microbial Technology·Chandra Shekhar Pundir, Rooma Devi
Jul 27, 2014·Analytical Biochemistry·Susan SadeghiMohammad Malekaneh
Mar 5, 2009·Journal of Pharmacological and Toxicological Methods·Hongyan LiBarbra J Sasu
May 10, 2016·Materials Science & Engineering. C, Materials for Biological Applications·N LavanyaG Ravi
Jun 28, 2016·Journal of Pharmaceutical and Biomedical Analysis·Rhishikesh ThakareYazen Alnouti
Jan 21, 2016·Biomedical Chromatography : BMC·Xi-Ling LiJun Zhe Min
Dec 4, 2012·Journal of Chromatographic Science·Mathrusri Annapurna MukthinuthalapatiChitaranjan Mohapatro
Jun 13, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kyung Mee KimRichard J Johnson
Aug 4, 2018·The Journal of Animal Ecology·David C EnsmingerMichael J Sheriff
Oct 31, 2009·Electrophoresis·Junge ZhangJoe P Foley
Jul 12, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xi LuoZeneng Cheng
Jul 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhiling ZhangKa Ming Ng
Nov 9, 2018·Frontiers in Chemistry·Md Nazibul IslamMohidus Samad Khan
Jun 1, 2016·Molecular Nutrition & Food Research·Paola Quifer-RadaRosa M Lamuela-Raventos
Feb 19, 2021·Epilepsia·Edward BeamerTobias Engel
May 1, 2021·3 Biotech·Nirmal Kant SharmaTek Chand Bhalla

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