Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas chromatography-mass spectrometry Application to polytraumatized patients

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Emilie MasThierry Durand

Abstract

Isoprostanes are a family of prostaglandin isomers produced from oxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. Quantification of F(2)-isoprostanes (F(2)-IsoPs) provides a good index of oxidative stress and allows non-invasive assessment of lipid peroxidation in vivo. Since "interferences peaks" at m/z 573 co-elute with d(4)-15-F(2t)-IsoP preferentially used, we propose a new GC-NICI-MS approach to quantify urinary F(2)-IsoPs by using 4(RS)-F(4t)-neuroprostane as the internal standard. This method was applied to quantify urinary F(2)-IsoPs excretion in healthy volunteers and polytraumatized patients. Our results showed a significant increase (p<0.0001) in urinary F(2)-IsoPs in polytraumatized patients compared with healthy volunteers (4.73+/-2.75 ng/mg vs. 0.811+/-0.359 ng/mg creatinine).

References

Dec 3, 1999·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J Helmersson, S Basu
Sep 14, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·E SchwedhelmJ C Frölich
Dec 9, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Erik S MusiekJason D Morrow
Jan 15, 2005·Antioxidants & Redox Signaling·Samar Basu, Johanna Helmersson
May 25, 2005·Injury·Marius Keel, Otmar Trentz
Nov 2, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kristina Claeson BohnstedtStaffan Schmidt
Sep 1, 2006·Journal of Lipid Research·John A LawsonJoshua Rokach
Dec 1, 2007·Injury·Andreas LenzWilliam G Cheadle

❮ Previous
Next ❯

Citations

Mar 29, 2012·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Christelle MachonJean-Paul Cristol
Aug 19, 2011·Chemical Reviews·Ginger L MilneL Jackson Roberts
Oct 30, 2008·Journal of Lipid Research·L Jackson Roberts, Ginger L Milne
May 13, 2014·Analytical and Bioanalytical Chemistry·Julia KuligowskiMáximo Vento
Mar 11, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Justyna AszykAgata Kot-Wasik
Apr 23, 2020·BMC Nephrology·Valerie OlivierOlivier Moranne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Trends in Endocrinology and Metabolism : TEM
D PraticòG A FitzGerald
Toxicology and Applied Pharmacology
Dejan MilatovicMichael Aschner
Toxicological Sciences : an Official Journal of the Society of Toxicology
Dejan MilatovicMichael Aschner
© 2022 Meta ULC. All rights reserved