Quantification of ZAP-70 mRNA by real-time PCR is a prognostic factor in chronic lymphocytic leukemia.

Journal of Cancer Research and Clinical Oncology
Yin-Hua WangWei Xu

Abstract

Chronic lymphocytic leukemia (CLL) is heterogeneous with respect to prognosis and clinical outcome. Mutational status of immunoglobulin heavy chain variable region (IGHV) appears to be a particularly strong prognostic marker, but it is difficult to perform in a routine clinical laboratory. ζ-chain-associated protein kinase 70 kDa (ZAP-70) protein detected by flow cytometry is a strong surrogate marker of IGHV mutational status; however, it suffers from the lack of standardization. We investigated whether ZAP-70 mRNA expression level can be a prognostic factor in CLL. Real-time quantitative polymerase chain reaction was used to analyze ZAP-70 mRNA expression from 102 CLL patients. The expression of ZAP-70 mRNA was significantly associated with Binet stage (P < 0.001), lactate dehydrogenase (P = 0.003), ZAP-70 protein (P = 0.018), IGHV mutational status (P = 0.038), and cytogenetic abnormality of del(17p13) or del(11q22.3) (P = 0.037) in CLL patients. According to receiver operating characteristic curve analysis for ZAP-70 mRNA and ZAP-70 protein, positive ZAP-70 mRNA (P = 0.006) was an adverse factor in determining the treatment free survival (TFS). In a multivariate Cox analysis of TFS, ZAP-70 mRNA is not ideal as an independen...Continue Reading

References

Jul 11, 2002·Annals of Hematology·T Hamblin
May 2, 2003·The New England Journal of Medicine·Marta CrespoEmili Montserrat
Jan 17, 2004·Lancet·Jenny A OrchardDavid G Oscier
Mar 8, 2006·European Journal of Haematology·Mark A CatherwoodH Denis Alexander
Aug 15, 2006·Cytometry. Part B, Clinical Cytometry·O G BestD G Oscier
Aug 15, 2006·Cytometry. Part B, Clinical Cytometry·M R SheikholeslamiM Albitar
Jun 22, 2011·Clinical and Experimental Medicine·Yin-Hua WangJian-Yong Li

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