Quantified analysis of cortical granule distribution and exocytosis of porcine oocytes during meiotic maturation and activation

Biology of Reproduction
W H WangB N Day

Abstract

Polyspermy is one of the unresolved problems that exist regarding pig oocytes matured and inseminated in vitro. Quantitative study of the changes in the cortical granule (CG) population in oocytes is essential for understanding the mechanism of how oocytes block polyspermic penetration and for developing the optimum conditions for in vitro maturation (IVM) and in vitro fertilization (IVF). The present study was conducted to quantify the CG distribution in pig oocytes during IVM and IVF by using fluorescein isothiocyanate-labeled peanut agglutinin with laser confocal microscopy. The results indicate that CGs are distributed in the cortex cytoplasm of oocytes at the germinal vesicle (GV) stage with a mean number of 33.8 +/- 7.3 CGs/100 microm2 of cortex. As nuclear maturation proceeded to metaphase I and metaphase II, CGs migrated to the cortex and formed a continuous monolayer under the oolemma. No distinct CG-free domain was observed in oocytes during maturation. The migration of CGs to the cortex continued during maturation, with an increased CG density after the GV stage. All oocytes penetrated by spermatozoa were activated and released CGs from ooplasm with an average residual number of 3.5 +/- 4.6 CGs/100 microm2 of cortex ...Continue Reading

Citations

Jan 4, 2001·Molecular Reproduction and Development·T Hoodbhoy, P Talbot
Mar 9, 2000·Journal of Reproductive Immunology·M R Barber, R A Fayrer-Hosken
Jun 14, 2000·Animal Reproduction Science·B N Day
Feb 10, 2011·Zygote : the Biology of Gametes and Early Embryos·Junhe HuHuayan Wang
Jul 29, 2011·Zygote : the Biology of Gametes and Early Embryos·Gabriel Ribas PereiraIrwin K M Liu
Oct 4, 2012·Animal : an International Journal of Animal Bioscience·L TůmováM Sedmíková
Oct 5, 2010·Reproduction, Fertility, and Development·Junping ChengDeshun Shi
Nov 18, 2011·Reproductive Biology and Endocrinology : RB&E·Min Liu
Apr 15, 2008·The Journal of Reproduction and Development·Koji YoshiokaAkira Onishi
Apr 2, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Yong-Hai LiWei-Hua Wang
Aug 10, 2012·Reproductive Biomedicine Online·Lei SunHou-Qi Liu
Feb 26, 2016·Reproductive Biomedicine Online·Sayyed Morteza Hosseini, Mohammad Hossein Nasr-Esfahani
Apr 14, 2009·Fertility and Sterility·Wei ShaQing-Yuan Sun
Jan 21, 2015·Animal Science Journal = Nihon Chikusan Gakkaihō·Rujun MaRong Rui
May 14, 2016·Zygote : the Biology of Gametes and Early Embryos·Rahul DuttaAngelika Schnieke
Jul 6, 2014·Zygote : the Biology of Gametes and Early Embryos·Junhe HuJian Zhao
Jan 22, 2014·Reproductive Biomedicine Online·Luna MaoFan Jin
Dec 9, 2014·Reproduction, Fertility, and Development·Hanne Skovsgaard PedersenPoul Hyttel
Jul 12, 2017·Zygote : the Biology of Gametes and Early Embryos·Mehdi Vafaye VallehPoul Hyttel
Feb 18, 2004·The Journal of Reproduction and Development·Qing-Yuan Sun, Takashi Nagai
Mar 10, 2004·The Journal of Reproduction and Development·Atsushi Asano, Koji Niwa
May 7, 1999·Molecular Reproduction and Development·W H WangB N Day
Feb 19, 2010·Biology of Reproduction·Andrej SusorMichal Kubelka
Jun 18, 2003·Microscopy Research and Technique·Wei-Hua WangGuang-Ming Wu
May 4, 2021·Veterinary Medicine and Science·Reina JochemsAnette K Krogenaes

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