Quantifying bacterial population dynamics in compost using 16S rRNA gene probes

Applied Microbiology and Biotechnology
Patrick D SchlossLarry P Walker

Abstract

Composting provides a dynamic setting for studying ecological topics such as succession, competition, and community stability in a relatively short period of time. This study used hierarchical small sub-unit-based rRNA gene probes to quantify the change in the relative abundance of phylogenetic groups common to compost in laboratory scale reactors. Bacterial 16S rRNA gene targets accounted for only 37% of all small subunit (SSU) rRNA genes initially, but increased to a maximum of 83% of the total at 84 h. The sum of rRNA genes detected using probes specific to Pseudomonas and low-G+C Gram-positive rRNA genes represented between 16% and 87% of the total. The lack of hybridization to the taxon-specific probes was most pronounced between 36 h and 60 h, when the pH was between 4.6 and 4.8. During this period the relative abundance of taxon-specific gene targets accounted for only 17-33% of the total bacterial rRNA gene targets. Pseudomonas-type 16S rRNA genes were the most abundant of the groups measured until 72 h. Those genes had their highest relative abundance at 12 h (78% of bacterial rRNA genes; 30% of all rRNA genes), after which time their relative abundance began to decline as the temperature increased. Prior to 72 h, 16S ...Continue Reading

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Citations

Sep 3, 2011·Environmental Technology·L T FontenelleL P Walker
Dec 23, 2006·Microbial Ecology·Diana R NemergutSteven K Schmidt
Sep 27, 2008·Microbial Ecology·Ingrid H Franke-WhittleHeribert Insam
Nov 23, 2006·Applied Microbiology and Biotechnology·Zh H YangY Sh Liu
Sep 28, 2007·Journal of Applied Microbiology·Y GuoC Deng
May 13, 2006·Biotechnology Advances·Y-H Percival ZhangJonathan R Mielenz
Oct 3, 2015·Carbohydrate Polymers·Mohit KumarSunil Khanna
May 3, 2011·Current Opinion in Microbiology·David B Wilson
Aug 3, 2017·Chemical Communications : Chem Comm·Lihao Guo, Akimitsu Okamoto

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