Quantifying calcium fluxes underlying calcium puffs in Xenopus laevis oocytes.

Cell Calcium
Luciana BrunoSilvina Ponce Dawson

Abstract

We determine the calcium fluxes through inositol 1,4,5-trisphosphate receptor/channels underlying calcium puffs of Xenopus laevis oocytes using a simplified version of the algorithm of Ventura et al. An analysis of 130 puffs obtained with Fluo-4 indicates that Ca2+ release comes from a region of width approximately 450 nm, that the release duration is peaked around 18 s and that the underlying Ca2+ currents range between 0.12 and 0.95 pA. All these parameters are independent of IP(3) concentration. We explore what distributions of channels that open during a puff, N(p), and what relations between current and number of open channels, I(N(p)), are compatible with our findings and with the distribution of puff-to-trigger amplitude ratio reported in Rose et al. To this end, we use simple "mean field" models in which all channels open and close simultaneously. We find that the variability among clusters plays an important role in shaping the observed puff amplitude distribution and that a model for which I(N(p)) approximately N(p) for small N(p) and I(N(p)) approximately N(p)(1/alpha) (alpha > 1) for large N(p), provides the best agreement. Simulations of more detailed models in which channels open and close stochastically show that...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G W De Young, J Keizer
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·L A BlatterE Ríos
Sep 3, 1998·Biophysical Journal·L T IzuC W Balke
Jul 10, 1999·The Journal of General Physiology·E RíosN Shirokova
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SwillensP Champeil
May 7, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Eduardo Ríos, Gustavo Brum
Aug 29, 2002·The Journal of General Physiology·S M BaylorW K Chandler
Feb 1, 2005·Biophysical Journal·Alejandra C VenturaSilvina Ponce Dawson
Mar 15, 2006·Biophysical Journal·Daniel FraimanSilvina Ponce Dawson
Aug 16, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Alejandra C VenturaSilvina Ponce Dawson
Sep 19, 2006·Biophysical Journal·Heather J RoseIan Parker
May 20, 2008·Journal of Theoretical Biology·Jeffrey R Groff, Gregory D Smith
Apr 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ian F Smith, Ian Parker

❮ Previous
Next ❯

Citations

May 13, 2011·Journal of Molecular Modeling·Irina BaranIoana Teodora Tofolean
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ward CapoenGiles E D Oldroyd
Nov 17, 2010·The Journal of General Physiology·Horia VaisDon-On Daniel Mak
Nov 17, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Guillermo Solovey, Silvina Ponce Dawson
Jan 21, 2012·Journal of Integrative Neuroscience·J RieraR Kawashima
Jun 14, 2012·PLoS Computational Biology·Sten RüdigerJian-Wei Shuai
Apr 30, 2014·PloS One·Estefanía PiegariSilvina Ponce Dawson
Jun 12, 2012·Cell Calcium·Ghanim UllahJohn E Pearson
May 11, 2012·Protein Science : a Publication of the Protein Society·Ambhighainath Ganesan, Jin Zhang
Feb 16, 2011·Biophysical Journal·Luis Diambra, Jonathan S Marchant
Sep 10, 2013·Biophysical Journal·Pengxing CaoJames Sneyd
May 27, 2014·Frontiers in Molecular Neuroscience·Kevin B Clark
Feb 23, 2010·PloS One·Guillermo Solovey, Silvina Ponce Dawson
Jan 15, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Ana CalabreseSilvina Ponce Dawson
Jan 31, 2014·Physical Biology·Daniel Fraiman, Silvina Ponce Dawson
Apr 2, 2019·Physical Review Letters·V VoorsluijsG Dupont
Aug 30, 2021·Biophysical Journal·Cecilia VillarruelSilvina Ponce Dawson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.