Quantifying cell-adhesion strength with micropipette manipulation: principle and application

Frontiers in Bioscience : a Journal and Virtual Library
Jin-Yu ShaoPeng Guo

Abstract

Quantifying cell-adhesion strength is of great importance in biology and medicine. Cell-adhesion strength can be characterized by separating two adherent cells and determining the force required to do so, or by measuring the lifetime of a receptor-ligand bond that mediates cell adhesion. To this end, several micropipette-based experimental techniques that operate at both cellular and molecular levels have been developed over the past few decades. In this review, we provide an overview of three of these techniques, i.e., the step-pressure technique (SPT), the biomembrane-force probe (BFP), and the micropipette-aspiration technique (MAT). More detailed discussion will be given about the requirements and applications of the MAT.

Citations

Dec 8, 2006·Annals of Biomedical Engineering·Jin-Yu Shao, Gang Xu
Dec 15, 2010·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Somyot Chirasatitsin, Adam J Engler
Jul 2, 2009·The Review of Scientific Instruments·Baoyu LiuJin-Yu Shao
Oct 25, 2014·PloS One·Rita SalánkiBálint Szabó
Mar 18, 2008·Cellular and Molecular Bioengineering·Da-Kang Yao, Jin-Yu Shao
Jan 1, 2009·Cellular and Molecular Bioengineering·Yong ChenJin-Yu Shao
May 13, 2019·ACS Biomaterials Science & Engineering·Fahad AlamKartik M Varadarajan
Sep 18, 2021·Scientific Reports·Rita Ungai-SalánkiRobert Horvath

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