Dec 22, 2014

Quantifying Cellular Capacity to Identify Gene Expression Designs With Reduced Burden

BioRxiv : the Preprint Server for Biology
Francesca CeroniTom Ellis

Abstract

Heterologous gene expression can be a significant burden to cells, consuming resources and causing decreased growth and stability. We describe here an in vivo monitor that tracks E. coli capacity changes in real-time and can be used to assay the burden synthetic constructs and their parts impose. By measuring capacity, construct designs with reduced burden can be identifiedand shown to predictably outperform less efficient designs, despite having equivalent expression outputs.

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Mentioned in this Paper

In Vivo
Gene Expression
Cellularization
Growth Retardation
Assay
Enzyme Stability
Escherichia coli
Entamoeba coli
Protein Expression

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