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Quantifying cellular capacity to identify gene expression designs with reduced burden

bioRxiv

Dec 22, 2014

Francesca CeroniTom Ellis

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Abstract

Heterologous gene expression can be a significant burden to cells, consuming resources and causing decreased growth and stability. We describe here an in vivo monitor that tracks E. coli capacity changes in real-time and can be used to assay the burden synthetic constructs and their par...read more

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  • Quantifying cellular capacity to identify gene expression designs with reduced burden

    bioRxiv

    Dec 22, 2014

    Francesca CeroniTom Ellis

    PMID: 990013110

    DOI: 10.1101/013110

    Abstract

    Heterologous gene expression can be a significant burden to cells, consuming resources and causing decreased growth and stability. We describe here an in vivo monitor that tracks E. coli capacity changes in real-time and can be used to assay the burden synthetic constructs and their par...read more

    11
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