Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis

Electrophoresis
John C SutherlandBetsy M Sutherland

Abstract

DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA.

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Citations

Apr 16, 2003·Analytical Biochemistry·Li DingRobert Dubrow
Apr 25, 2003·Forensic Science International : Synergy·Masahiro MukaidaHiroko Kimura
Aug 21, 2003·Free Radical Biology & Medicine·Betsy M SutherlandJacques Laval
Jun 28, 2002·Nucleic Acids Research·Alexandros G GeorgakilasBetsy M Sutherland
Oct 21, 2004·Nucleic Acids Research·Alexandros G GeorgakilasBetsy M Sutherland
Aug 27, 2002·Photochemistry and Photobiology·John C Sutherland
Apr 28, 2005·Experimental Dermatology·Steen LisbyHans Christian Wulf
Jul 17, 2004·Free Radical Biology & Medicine·Paula V BennettBetsy M Sutherland
Aug 20, 2005·Free Radical Biology & Medicine·Paula V BennettBetsy M Sutherland
Jul 24, 2008·Dose-response : a Publication of International Hormesis Society·Antone L Brooks, Lezlie Couch
Jul 16, 2010·Technology in Cancer Research & Treatment·Alex Goldberg, Boris Rubinsky
May 15, 2002·Radiation Research·Betsy M SutherlandJacques Laval
Jan 27, 2006·Radiation Research·Megumi Hada, Betsy M Sutherland
Sep 5, 2002·Photodermatology, Photoimmunology & Photomedicine·Betsy M SutherlandR W Gange
Jun 10, 2003·Journal of Radiation Research·Betsy M SutherlandAlan Gewirtz
Nov 16, 2002·Science·Robert J Linhardt, Toshihiko Toida
Nov 18, 2021·International Journal of Radiation Biology·Houda KacemMarie-Catherine Vozenin

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