Quantifying peptides in isotopically labeled protease digests by ion mobility/time-of-flight mass spectrometry

Analytical Chemistry
Jennifer M KindyDavid E Clemmer

Abstract

Ion mobility/time-of-flight techniques have been used to analyze mixtures of isotopically labeled peptides. The isotopic labels were generated by treatment of peptides with N-acetoxysuccinimide (or the deuterated analogue), which results in acetylation (or deuterioacetylation) of the primary amines (i.e., the N-terminus and lysine residues). The relative concentrations of a peptide in each sample are determined by comparing the peak intensities for isotopic pairs. An important consideration is that as mixtures become increasingly complex, isotopic pairs of peaks may overlap with other peaks in the mass spectrum. The influence of the acetyl and deuterioacetyl groups on the mobilities of peptides is considered. The coincidence in mobilities of isotopic pairs provides a means of distinguishing isotopic pairs from other isobaric interferences.

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Citations

Aug 7, 2013·Journal of Structural and Functional Genomics·Eric D MerkleyJoshua N Adkins
Feb 21, 2013·Journal of the American Society for Mass Spectrometry·Eric D MerkleyJoshua N Adkins
Jun 1, 2005·Journal of the American Society for Mass Spectrometry·Xiang ZhangFred E Regnier
Mar 1, 2006·Journal of the American Society for Mass Spectrometry·Victor V Laiko
Jan 28, 2003·Current Opinion in Chemical Biology·Salvatore Sechi, Yoshiya Oda
Nov 5, 2002·Current Opinion in Chemical Biology·Wenjun Mo, Barry L Karger
Nov 30, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Brandon T RuotoloDavid H Russell
Mar 21, 2008·Molecular & Cellular Proteomics : MCP·Zhiyin XunDavid E Clemmer
Jul 21, 2006·Proteome Science·Frédéric Delom, Eric Chevet
Jan 18, 2008·Journal of Mass Spectrometry : JMS·Abu B KanuHerbert H Hill
Mar 29, 2014·Rapid Communications in Mass Spectrometry : RCM·Robert M SturmLingjun Li
Dec 16, 2016·Journal of the American Society for Mass Spectrometry·Julia L Kaszycki, Alexandre A Shvartsburg
Jul 19, 2002·Journal of Mass Spectrometry : JMS
May 9, 2009·European Journal of Mass Spectrometry·David P SmithAlison E Ashcroft
Jan 13, 2006·Mass Spectrometry Reviews·Lloyd D FrickerFa-Yun Che
Apr 24, 2020·Analytical and Bioanalytical Chemistry·Yifei WangPei Chen
Mar 15, 2011·Organic & Biomolecular Chemistry·Fumihiro Ito, Kentaro Yamaguchi
Sep 2, 2008·Journal of Agricultural and Food Chemistry·Dion M A M LuykxHans Bouwmeester
Aug 15, 2002·Analytical Chemistry·Roujian ZhangFred E Regnier
Jul 16, 2004·Journal of Proteome Research·Samir Julka, Fred Regnier
Dec 31, 2003·Analytical Chemistry·Benjamin J CargileJames L Stephenson
Apr 19, 2002·Journal of the American Chemical Society·Brandon T RuotoloDavid H Russell
Aug 9, 2007·Journal of Proteome Research·Zhiyin XunDavid E Clemmer

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