Quantifying the cellular uptake of semiconductor quantum dot nanoparticles by analytical electron microscopy

Journal of Microscopy
Nicole HondowAndy Brown

Abstract

Semiconductor quantum dot nanoparticles are in demand as optical biomarkers yet the cellular uptake process is not fully understood; quantification of numbers and the fate of internalized particles are still to be achieved. We have focussed on the characterization of cellular uptake of quantum dots using a combination of analytical electron microscopies because of the spatial resolution available to examine uptake at the nanoparticle level, using both imaging to locate particles and spectroscopy to confirm identity. In this study, commercially available quantum dots, CdSe/ZnS core/shell particles coated in peptides to target cellular uptake by endocytosis, have been investigated in terms of the agglomeration state in typical cell culture media, the traverse of particle agglomerates across U-2 OS cell membranes during endocytosis, the merging of endosomal vesicles during incubation of cells and in the correlation of imaging flow cytometry and transmission electron microscopy to measure the final nanoparticle dose internalized by the U-2 OS cells. We show that a combination of analytical transmission electron microscopy and serial block face scanning electron microscopy can provide a comprehensive description of the internalizati...Continue Reading

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Citations

Jul 8, 2017·Microscopy Research and Technique·Pabel Cervantes-AvilésGermán Cuevas-Rodríguez
Dec 29, 2020·Journal of Colloid and Interface Science·Laura I FitzGerald, Angus P R Johnston

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