Quantifying the effect of electric current on cell adhesion studied by single-cell force spectroscopy

Biointerphases
Leena JaatinenLászló Demkó

Abstract

This study presents the effect of external electric current on the cell adhesive and mechanical properties of the C2C12 mouse myoblast cell line. Changes in cell morphology, viability, cytoskeleton, and focal adhesion structure were studied by standard staining protocols, while single-cell force spectroscopy based on the fluidic force microscopy technology provided a rapid, serial quantification and detailed analysis of cell adhesion and its dynamics. The setup allowed measurements of adhesion forces up to the μN range, and total detachment distances over 40 μm. Force-distance curves have been fitted with a simple elastic model including a cell detachment protocol in order to estimate the Young's modulus of the cells, as well as to reveal changes in the dynamic properties as functions of the applied current dose. While the cell spreading area decreased monotonously with increasing current doses, small current doses resulted only in differences related to cell elasticity. Current doses above 11 As/m(2), however, initiated more drastic changes in cell morphology, viability, cellular structure, as well as in properties related to cell adhesion. The observed differences, eventually leading to cell death toward higher doses, might o...Continue Reading

References

Jul 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M R ChoD E Golan
Mar 30, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M R ChoD E Golan
May 26, 1999·The Journal of Membrane Biology·F HofmannC Jassoy
Nov 26, 1999·Journal of Cellular Biochemistry·H SauerM Wartenberg
Dec 28, 1999·Journal of Biomechanics·R M Hochmuth
Oct 12, 2000·Biochemical and Biophysical Research Communications·F J AlenghatD E Ingber
Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·H U SimonF Levi-Schaffer
Dec 12, 2002·Cells, Tissues, Organs·Martin Benoit, Hermann E Gaub
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Charles R KeeseIvar Giaever
Jun 11, 2005·The Journal of Physiology·Christian StockAlbrecht Schwab
Jun 24, 2005·Journal of Biomechanical Engineering·Emiel A G PeetersFrank P T Baaijens
Jul 1, 2005·Physiological Reviews·Colin D McCaigMin Zhao
Jul 8, 2005·Molecular Biology of the Cell·Nathan D GallantAndrés J García
Apr 28, 2007·Annual Review of Biomedical Engineering·Paul A Janmey, Christopher A McCulloch
Jul 27, 2007·Connective Tissue Research·Pen-Hsiu Grace ChaoJeannette Chloë Bulinski
Aug 8, 2007·Journal of Cellular Physiology·Allison L Berrier, Kenneth M Yamada
Nov 20, 2009·Journal of Cell Science·Colin D McCaigAnn M Rajnicek
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Michael GabiJanos Vörös
Apr 23, 2010·Biointerphases·Gilles WederMartha Liley
Jun 15, 2010·Colloids and Surfaces. B, Biointerfaces·Michael GabiJanos Vörös
Jul 14, 2010·Journal of Cellular Biochemistry·David A LeeDan L Bader
Nov 9, 2010·Biomedical Microdevices·Orane Guillaume-GentilJanos Vörös
Feb 2, 2011·PloS One·Ranjani K ParadiseKrystyn J Van Vliet
May 24, 2011·The International Journal of Biochemistry & Cell Biology·Geum-Hwa LeeHyung-Ryong Kim
Jun 26, 2012·Chemistry & Biology·Kristin Grant Beaumont, Milan Mrksich
Aug 10, 2012·Tissue Engineering. Part B, Reviews·Richard BalintSarah H Cartmell
May 22, 2013·Tissue Engineering. Part B, Reviews·Anna V TaubenbergerDaniel J Muller
Jul 12, 2013·PloS One·Bruno PontesH Moysés Nussenzveig
Feb 25, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Carina GonnermannClemens M Franz
Aug 8, 2015·International Journal of Molecular Sciences·Amelia Ahmad Khalili, Mohd Ridzuan Ahmad
Aug 15, 2015·Oxidative Medicine and Cellular Longevity·Sébastien SartYan Li

❮ Previous
Next ❯

Citations

Sep 10, 2016·Nanotechnology·Dario OssolaMassimo Vassalli
Nov 27, 2020·Brain Sciences·Farah Amna Othman, Suat Cheng Tan
Apr 22, 2017·The International Journal of Artificial Organs·Hadis EghbaliManuela T Raimondi
Mar 17, 2020·Biosensors & Bioelectronics·Jong Min LeeBong Geun Chung
Apr 4, 2021·Cells·Ashwini ShindeTuhin Subhra Santra

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.